Biophysical Journal 52: 145-153 (1987)
© 1987 the Biophysical Society

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Hydrophobic molecules in lecithin-water systems. I. Formation of reversed hexagonal phases at high and low water contents.

Department of Physical Chemistry, University of Umeå, Sweden.

ABSTRACT

The system dioleoylphosphatidylcholine (DOPC)-n-dodecane-2H2O was investigated with different nuclear magnetic resonance (NMR) techniques: (a) a tentative phase diagram was determined by 2H- and 31P-NMR, (b) translational diffusion coefficients were determined for the three components with the pulsed magnetic field gradient NMR technique, and (c) order parameters for perdeuterated n-dodecane were obtained by 2H-NMR. n-Dodecane induces the formation of reversed hexagonal (HII) phases at low and high water concentrations, and cubic phases at low water contents. The translational diffusion coefficients of n-dodecane in a cubic phase with 6 mol water per mol DOPC, and in an HII phase with 48 mol water per mol DOPC, were just approximately 2.5 times lower than in pure dodecane. Perdeuterated dodecane gave large quadrupole splittings in a lamellar phase, much smaller in an HII phase at low water contents, and a narrow single peak in an HII phase at high water contents. This latter observation indicates that a large fraction of the dodecane molecules is located in separate regions between the water cylinders. Our results support the model given by Gruner concerning the aggregation of membrane lipids in the presence of hydrophobic molecules.

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