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Biophysical Journal 53: 145-152 (1988)
© 1988 the Biophysical Society

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23Na-nuclear magnetic resonance investigation of gramicidin-induced ion transport through membranes under equilibrium conditions.

D C Buster, J F Hinton, F S Millett and D C Shungu

Department of Chemistry/Biochemistry, University of Arkansas, Fayetteville 72701.

ABSTRACT

A technique for investigating the gramicidin-facilitated transport of Na+ ions across lipid bilayers of large unilamellar vesicles under the condition of ionic equilibrium has been developed using a combination of heat incubation of the gramicidin with the vesicles and 23Na-nuclear magnetic resonance (NMR) spectroscopy. Isolation of the two 23Na-NMR signals from the intra- and extravesicular Na+ with the shift reagent, dysprosium (III) tripolyphosphate, allows the equilibrium flux of Na+ through the gramicidin channels to be detected and treated as a two-site exchange process. This study indicates that the transport of Na+ through gramicidin channels is second order with respect to the gramicidin concentration.




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J. B. Jordan, P. L. Easton, and J. F. Hinton
Effects of Phenylalanine Substitutions in Gramicidin A on the Kinetics of Channel Formation in Vesicles and Channel Structure in SDS Micelles
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[Abstract] [Full Text] [PDF]

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A. O'Connell, R. Koeppe 2nd, and O. Andersen
Kinetics of gramicidin channel formation in lipid bilayers: transmembrane monomer association
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Copyright © 1988 by the Biophysical Society.