Biophysical Journal 53: 415-423 (1988)
© 1988 the Biophysical Society

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Passive interaction between sliding filaments in the osmotically compressed skinned muscle fibers of the frog.

Department of Physiology, School of Medicine, Teikyo University Kaga, Tokyo, Japan.

ABSTRACT

Shortening and lengthening velocities, instantaneous stiffness, and tension transients after stretch were measured in compressed muscle fibers from the frog in the presence or absence of polyvinylpyrrolidone (PVP K30) or Dextran T70. Both shortening and lengthening velocities clearly decreased with the concentration of polymer. In the presence of polymer, "passive" stiffness was observed in relaxing solution depending on fiber diameter, and stiffness increased further by activation. This increase by activation above "passive" stiffness was nearly constant in the wide range of polymer concentrations. These active and "passive" stiffnesses were found to be dependent on sarcomere length. The stiffness of a compressed rigor fiber was indicated to be composed of constant rigor stiffness and a variable "passive" one. The tension transient after stretch in a compressed active or rigor fiber was also indicated to be composed of two kinds of transients. The above results suggest that (a) there exist two kinds of interactions in parallel in a compressed active or rigor fiber: one active or rigor and another "passive" between sliding filaments, and (b) the decrease in shortening velocity in a compressed fiber may be brought about by this "passive" interaction.

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