| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 53: 635-639 (1988)
© 1988 the Biophysical Society
Department of Physiology-Anatomy, University of California, Berkeley 94720.
ABSTRACT
The Ca2+-binding kinetics of fura-2 and azo-1 were studied using temperature-jump relaxation methods. In 140 mM KCl at 20 degrees C, the association and dissociation rate constants for fura-2 were 6.02 x 10(8) M-1s-1 and 96.7 s-1, respectively. The fura-2 kinetics were insensitive to pH over the range 7.4 to 8.4. Azo-1 was studied in 140 mM KCl, at pH 7.4, at 10 degrees and 20 degrees C. At 10 degrees C, azo-1 exhibited association and dissociation rate constants of 1.43 x 10(8) M-1s-1 and 777.9 s-1, respectively; while at 20 degrees C, the corresponding values were 3.99 x 10(8) M-1s-1 and 1,177 s-1. The kinetic results demonstrate that fura-2 and azo-1 are well suited to monitoring rapid changes in intracellular [Ca2+].
This article has been cited by other articles:
 |
|
 |
|
  M. Patterson, J. Sneyd, and D. D. Friel Depolarization-induced Calcium Responses in Sympathetic Neurons: Relative Contributions from Ca2+ Entry, Extrusion, ER/Mitochondrial Ca2+ Uptake and Release, and Ca2+ Buffering J. Gen. Physiol., January 1, 2007; 129(1): 29 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Glass, T. M. Pocock, F. E. Curry, and D. O. Bates Cytosolic Ca2+ concentration and rate of increase of the cytosolic Ca2+ concentration in the regulation of vascular permeability in Rana in vivo J. Physiol., May 1, 2005; 564(3): 817 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Millar, R. S. Zucker, G. C. R. Ellis-Davies, M. P. Charlton, and H. L. Atwood Calcium Sensitivity of Neurotransmitter Release Differs at Phasic and Tonic Synapses J. Neurosci., March 23, 2005; 25(12): 3113 - 3125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ursu, R. P. Schuhmeier, and W. Melzer Voltage-controlled Ca2+ release and entry flux in isolated adult muscle fibres of the mouse J. Physiol., January 15, 2005; 562(2): 347 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. McHugh and J. L. Kenyon An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell Am J Physiol Cell Physiol, February 1, 2004; 286(2): C342 - C348. [Abstract] [Full Text]
|
|
|
|
|
|
R.P. Schuhmeier and W. Melzer Voltage-dependent Ca2+ Fluxes in Skeletal Myotubes Determined Using a Removal Model Analysis J. Gen. Physiol., December 29, 2003; 123(1): 33 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Schuhmeier, B. Dietze, D. Ursu, F. Lehmann-Horn, and W. Melzer Voltage-Activated Calcium Signals in Myotubes Loaded with High Concentrations of EGTA Biophys. J., February 1, 2003; 84(2): 1065 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ouanounou, L. Zhang, M. P. Charlton, and P. L. Carlen Differential Modulation of Synaptic Transmission by Calcium Chelators in Young and Aged Hippocampal CA1 Neurons: Evidence for Altered Calcium Homeostasis in Aging J. Neurosci., February 1, 1999; 19(3): 906 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Fitzsimmons, J. A. McRoberts, K. H. Tachiki, and S. J. Pandol Acyl-Coenzyme A Causes Ca2+ Release in Pancreatic Acinar Cells J. Biol. Chem., December 12, 1997; 272(50): 31435 - 31440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, M. Tymianski, O. T. Jones, and M. Nedergaard Impact of Cytoplasmic Calcium Buffering on the Spatial and Temporal Characteristics of Intercellular Calcium Signals in Astrocytes J. Neurosci., October 1, 1997; 17(19): 7359 - 7371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Abdel-Hamid and M. Tymianski Mechanisms and Effects of Intracellular Calcium Buffering on Neuronal Survival in Organotypic Hippocampal Cultures Exposed to Anoxia/Aglycemia or to Excitotoxins J. Neurosci., May 15, 1997; 17(10): 3538 - 3553. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
