Biophysical Journal 53: 1015-1019 (1988)
© 1988 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kinosita, K Articles by Ikegami, A Search for Related Content PubMed PubMed Citation Articles by Kinosita, K, Jr Articles by Ikegami, A

Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope.

Institute of Physical and Chemical Research, Saitama, Japan.

ABSTRACT

Controlled permeability can be conferred to cell membranes by exposing cells to a microsecond electric pulse of sufficient intensity (electroporation). By constructing a fluorescence microimaging system with a submicrosecond time resolution we have been able to resolve temporally and spatially the events in a single cell under a microsecond electric pulse. An enormous membrane conductance, corresponding to a loss of 0.01-0.1% of the membrane area, was observed in those membrane regions where the transmembrane potential induced by the electric pulse exceeded a critical value. The conductance decreased to a low level in a submillisecond after the pulse, leaving a moderately electroporated cell.

This article has been cited by other articles:

				K. C. Smith and J. C. Weaver Active Mechanisms Are Needed to Describe Cell Responses to Submicrosecond, Megavolt-per-Meter Pulses: Cell Models for Ultrashort Pulses Biophys. J., August 15, 2008; 95(4): 1547 - 1563. [Abstract] [Full Text] [PDF]

				D. A. Zaharoff, J. W. Henshaw, B. Mossop, and F. Yuan Mechanistic Analysis of Electroporation-Induced Cellular Uptake of Macromolecules Experimental Biology and Medicine, January 1, 2008; 233(1): 94 - 105. [Abstract] [Full Text] [PDF]

				K. A. Riske and R. Dimova Electric Pulses Induce Cylindrical Deformations on Giant Vesicles in Salt Solutions Biophys. J., September 1, 2006; 91(5): 1778 - 1786. [Abstract] [Full Text] [PDF]

				W. Frey, J. A. White, R. O. Price, P. F. Blackmore, R. P. Joshi, R. Nuccitelli, S. J. Beebe, K. H. Schoenbach, and J. F. Kolb Plasma Membrane Voltage Changes during Nanosecond Pulsed Electric Field Exposure Biophys. J., May 15, 2006; 90(10): 3608 - 3615. [Abstract] [Full Text] [PDF]

				K. A. Riske and R. Dimova Electro-Deformation and Poration of Giant Vesicles Viewed with High Temporal Resolution Biophys. J., February 1, 2005; 88(2): 1143 - 1155. [Abstract] [Full Text] [PDF]

				A. Takahashi, P. Camacho, J. D. Lechleiter, and B. Herman Measurement of Intracellular Calcium Physiol Rev, October 1, 1999; 79(4): 1089 - 1125. [Abstract] [Full Text] [PDF]

				F. BOBANOVIC, M. D. BOOTMAN, M. J. BERRIDGE, N. A. PARKINSON, and P. LIPP Elementary [Ca2+]i signals generated by electroporation functionally mimic those evoked by hormonal stimulation FASEB J, February 1, 1999; 13(2): 365 - 376. [Abstract] [Full Text]

				J. A. Lundqvist, F. Sahlin, M. A. I. Aberg, A. Stromberg, P. S. Eriksson, and O. Orwar Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes PNAS, September 1, 1998; 95(18): 10356 - 10360. [Abstract] [Full Text] [PDF]