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Biophysical Journal 54: 357-363 (1988)
© 1988 the Biophysical Society

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Sarcoballs: direct access to sarcoplasmic reticulum Ca2+-channels in skinned frog muscle fibers.

P Stein and P Palade

Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston 77550.

ABSTRACT

In skeletal muscle, twitch contraction is caused by the rapid release of Ca2+ from the sarcoplasmic reticulum (SR) (Endo, M. 1977. Physiol. Rev. 57:71-108) via Ca2+ conducting channels in the SR membrane (Smith, J. S., R. Coronado, and G. Meissner, 1985. Nature (Lond.). 316:446-449; Suarez-Isla, B. A., G. Orozco, P. F. Heller, and J. P. Froehlich. 1986. Proc. Natl. Acad. Sci. USA. 83:7741-7745). To facilitate study of these and other intracellular channels, we have developed a method which allows direct patch-clamp recording of currents through SR channels in native membrane. The Ca2+-release channel studied using this method exhibits two predominant conductance levels (80-100 pS and 120-160 pS), conducts Ca2+ preferentially over K+ (PCa/Pk = 6.5), is highly voltage sensitive, blocked on one side by ruthenium red (1 microM), and displays enhanced activity in the presence of caffeine (5 mM). Studied in skinned fibers, this channel appears fundamentally similar to homologous channels from isolated rabbit SR incorporated into bilayers, with some distinct differences.




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D. Gillespie and M. Fill
Intracellular Calcium Release Channels Mediate Their Own Countercurrent: The Ryanodine Receptor Case Study
Biophys. J., October 15, 2008; 95(8): 3706 - 3714.
[Abstract] [Full Text] [PDF]


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