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Biophysical Journal 54: 407-410 (1988)
© 1988 the Biophysical Society

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Gibbs-Donnan ratio and channel conductance of Tetrahymena cilia in mixed solution of K+ and Ca2+.

Y Oosawa and M Kasai

Department of Biophysical Engineering, Faculty of Engineering Sciences, Osaka University, Japan.

ABSTRACT

A single cation-channel from Tetrahymena cilia was incorporated into planar lipid bilayers. This channel was voltage-independent and is permeable to K+ and Ca2+. In the experiments with mixed solutions where the concentrations of K+ and Ca2+ were varied, the single-channel conductance was found to be influenced by the Gibbs-Donnan ratio. The data are explained by assuming that the binding sites of this channel were always occupied by two potassium ions or one calcium ion under the present experimental conditions (5 mM-90 mM K+ and 0.5 mM-35 mM Ca2+) and these bound cations determined the channel conductivity.






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Copyright © 1988 by the Biophysical Society.