Biophysical Journal 54: 595-602 (1988)
© 1988 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kuwahara, M Articles by Verkman, A S Search for Related Content PubMed PubMed Citation Articles by Kuwahara, M Articles by Verkman, A S

Rapid development of vasopressin-induced hydroosmosis in kidney collecting tubules measured by a new fluorescence technique.

Department of Medicine, University of California, San Francisco 94143.

ABSTRACT

The pre-steady-state kinetics of the vasopressin-induced increase in collecting tubule osmotic water permeability (Pf) has been measured by a new fluorescence technique. Isolated cortical collecting tubules (CCT) from rabbit kidney were perfused with physiological buffers containing the impermeant fluorophores fluorescein sulfonate (FS) and pyrenetetrasulfonic acid (PTSA). Tubules were subject to a 120 mOsm bath-to-lumen osmotic gradient in the presence and absence of 250 microU/ml vasopressin. The magnitude of transepithelial volume flow was determined from the self-quenching of FS, or from the ratio of PTSA/FS fluorescence, measured at 380 nm excitation and 420 +/- 10 nm (PTSA) and greater than 530 nm (FS) emission wavelengths. Pf was calculated from the magnitude of transepithelial volume flow, lumen and bath osmolarities, lumen perfusion rate, and tubule geometry. The instrument response time for a change in bath osmolality was less than 3 s. At 37 degrees C, CCT Pf was (in units of cm/s x 10(4] 13 +/- 2 (mean +/- SE, 16 tubules) before, and 227 +/- 10 after addition of vasopressin to the bath. CCT Pf began to increase in 23 +/- 3 s after vasopressin addition and was half-maximal after 186 +/- 20 s. At 23 degrees C, Pf was 9 +/- 1 (seven tubules) before, and 189 +/- 12 after vasopressin addition. Pf began to increase in 40 +/- 4 s and was half-maximal after 195 +/- 35 s. After vasopressin removal from the bath, Pf decreased to its baseline value with a half-time of 14 min.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				A. Banan, J. Z. Fields, D. A. Talmage, Y. Zhang, and A. Keshavarzian PKC-{beta}1 mediates EGF protection of microtubules and barrier of intestinal monolayers against oxidants Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G833 - G847. [Abstract] [Full Text] [PDF]

				K. S. Wang, T. Ma, F. Filiz, A. S. Verkman, and J. A. Bastidas Colon water transport in transgenic mice lacking aquaporin-4 water channels Am J Physiol Gastrointest Liver Physiol, August 1, 2000; 279(2): G463 - G470. [Abstract] [Full Text] [PDF]

				A. S. Verkman, M. A. Matthay, and Y. Song Aquaporin water channels and lung physiology Am J Physiol Lung Cell Mol Physiol, May 1, 2000; 278(5): L867 - L879. [Abstract] [Full Text] [PDF]

				O. Seksek, J. Biwersi, and A. S. Verkman Direct Measurement of trans-Golgi pH in Living Cells and Regulation by Second Messengers J. Biol. Chem., March 10, 1995; 270(10): 4967 - 4970. [Abstract] [Full Text] [PDF]