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Biophysical Journal 55: 649-654 (1989)
© 1989 the Biophysical Society

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Ultrasonic Cole-Cole diagram for solutions and application to alpha-chymotrypsin.

R Cerf, S T Salehi and D Rogez

Laboratoire de Spectrométrie et d'Imagerie Ultrasonores, Université Louis Pasteur, Strasbourg, France.

ABSTRACT

Deconvolution of ultrasonic data into single relaxations is rarely feasible when only the absorption or the velocity of the waves is measured. Here we use both series of data to construct a Cole-Cole diagram for a solution. When applied to alpha-chymotrypsin, this method shows two relaxations that are well separated on the time scale, a result that will help simplify analyses of the ultrasonic data for this enzyme.






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Copyright © 1989 by the Biophysical Society.