Biophysical Journal 55: 673-676 (1989)
© 1989 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Milne, R K Articles by Edeson, R O Search for Related Content PubMed PubMed Citation Articles by Milne, R K Articles by Edeson, R O

Estimation of single channel kinetic parameters from data subject to limited time resolution.

Department of Mathematics, University of Western Australia, Nedlands.

ABSTRACT

The limited responsiveness of single-channel recording systems results in some brief events not being detected, and if this is ignored parameter estimation from the observed data will be biased. Statistical methods of correcting for this limited time resolution in a two-state Markov model have been proposed by Neher (1983. J. Physiol. (Lond.). 339:663-678) and by Colquhoun and Sigworth (1983. Single Channel Recording. 191-263). However, a numerical study by Blatz and Magleby (1986. Biophys. J. 49:967-980) indicated differences of 3-40% in the corrected values given by the two techniques. Here we explain why Neher's method produces biased results and the Colquhoun and Sigworth approach, which is no more difficult, provides reasonably accurate estimates.

This article has been cited by other articles:

				L. Moffatt Estimation of Ion Channel Kinetics from Fluctuations of Macroscopic Currents Biophys. J., July 1, 2007; 93(1): 74 - 91. [Abstract] [Full Text] [PDF]