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Biophysical Journal 55: 1025-1031 (1989)
© 1989 the Biophysical Society

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Fluorescence depolarization study of lamellar liquid crystalline to inverted cylindrical micellar phase transition of phosphatidylethanolamine.

K H Cheng

Department of Physics, Texas Tech University, Lubbock 79409.

ABSTRACT

The orientational order and rotational dynamics of 2-[3-(diphenyl-hexatrienyl) propanoyl]-3-palmitoyl-L-alpha- phosphatidylcholine (DPH-PC) embedded in dioleoylphosphatidyl-ethanolamine (DOPE) were studied by fluorescence depolarization technique. Upon increasing the temperature, the calculated wobbling diffusion constant D perpendicular of the fluorescent probe was found to decrease at the lamellar (L alpha) to inverted cylindrical (H II) phase transition (10 degrees C). This suggested that the increased gauche rotamers of the alkene chains in the HII phase imposes a constraint in the wobbling motion of the fluorophore. The calculated ratio of order parameter in the L alpha phase to that in the HII phase was 1.7 and different from the theoretical value of 2.0 as predicted from the change in packing symmetry. This result can be explained by a slightly higher local order parameter of the fluorophore or by the fast rotational diffusion motion of the fluorophore around the symmetry axis of the cylindrical tubes in the HII phase.




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B. Cannon, G. Heath, J. Huang, P. Somerharju, J. A. Virtanen, and K. H. Cheng
Time-Resolved Fluorescence and Fourier Transform Infrared Spectroscopic Investigations of Lateral Packing Defects and Superlattice Domains in Compositionally Uniform Cholesterol/Phosphatidylcholine Bilayers
Biophys. J., June 1, 2003; 84(6): 3777 - 3791.
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Copyright © 1989 by the Biophysical Society.