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Biophysical Journal 56: 115-119 (1989)
© 1989 the Biophysical Society

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Incorporation of ionic channels from yeast plasma membranes into black lipid membranes.

F Gómez-Lagunas, A Peña, A Liévano and A Darszon

Instituto de Fisiología Celular, Universidad Nacional Autónoma de México.

ABSTRACT

Recently, patch-clamping of yeast protoplasts has revealed the presence of plasma membrane K+ channels (Gustin, M. C., B. Martinac, Y. Saimi, M. R. Culberston, and C. Kung. 1986. Science (Wash. DC). 233:1195-1197). In this work we show that fusion of purified plasma membranes into planar bilayers allows the study of the yeast channels. The main cationic conductances detected were of 64 and 116 pS, however, larger and smaller conductances have been observed. The two main conductances were sensitive to the K+ channels blockers tetraethylammonium (TEA+) and Ba2+. Bionic experiments indicated that both conductances were K+ selective.






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Copyright © 1989 by the Biophysical Society.