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Biophysical Journal 56: 447-452 (1989)
© 1989 the Biophysical Society

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Phosphorus-31 nuclear magnetic resonance spectroscopy of toad retina.

D V Apte, Y Koutalos, D K McFarlane, M J Dawson and T G Ebrey

Department of Physiology and Biophysics, University of Illinois, Urbana 61801.

ABSTRACT

Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spectra of whole retinae. The intracellular pH was determined to be 7.27 +/- 0.06 at 4 degrees C and the intracellular MgNTP/NTP ratio was at least 0.77. These results are consistent with those reported by other techniques, and they show that 31P-NMR spectroscopy can be used for noninvasively and quantitatively studying the metabolism of living toad retinae, and for monitoring its changes over time.






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Copyright © 1989 by the Biophysical Society.