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Biophysical Journal 56: 459-463 (1989)
© 1989 the Biophysical Society

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Charge motion in MbCO crystals after flash photolysis.

I E Iben, L Keszthelyi, D Ringe and G Váró

Department of Physics, University of Illinois, Urbana 61801.

ABSTRACT

Charge motion accompanying the dissociation and recombination of carbon monoxide to oriented myoglobin crystals has been observed. The magnitude of the electrical signals detected after photodissociation by electrodes on either side of MbCO crystals of type A is consistent with the x-ray data showing that the doubly charged iron ion lies in the mean heme plane when a ligand is bound and moves out of the plane when deligated. Beyond 10 ms, the time development of the electrical signal is consistent with the kinetics observed optically after flash photolysis on the same crystals.




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V. , T.-y. Teng, T. Ursby, C. Pradervand, Z. Ren, S.-i. Adachi, W. Schildkamp, D. Bourgeois, M. Wulff, and K. Moffat
Photolysis of the Carbon Monoxide Complex of Myoglobin: Nanosecond Time-Resolved Crystallography
Science, December 6, 1996; 274(5293): 1726 - 1729.
[Abstract] [Full Text]




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Copyright © 1989 by the Biophysical Society.