Biophysical Journal 56: 465-475 (1989)
© 1989 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Ortega, J. K. E. Articles by Keanini, R. G. Search for Related Content PubMed Articles by Ortega, J. K. E. Articles by Keanini, R. G.

In vivo creep and stress relaxation experiments to determine the wall extensibility and yield threshold for the sporangiophores of phycomyces

Department of Mechanical Engineering, University of Colorado at Denver, Denver, Colorado 80204-5300

ABSTRACT

The pressure probe was used to conduct in vivo creep and in vivo stress relaxation experiments on the sporangiophores of Phycomyces blakesleeanus. The in vivo creep and in vivo stress relaxation methods are compared with respect to their utility for determining the irreversible wall extensibility and the yield threshold. The results of the in vivo stress relaxation experiments demonstrate that the growth usually does not cease when the external water supply is removed, and the turgor pressure does not decay for hours afterwards. A successful stress relaxation experiment requires that the cell enlargement rate (growth rate) be zero during the turgor pressure decay. In a few experiments, the growth rate was zero during the turgor pressure decay. However, in general only the yield threshold could be determined.

In vivo creep experiments proved to be easier to conduct and more useful in determining values for both the irreversible wall extensibility and the yield threshold. The results of the in vivo creep experiments demonstrate that small steps-up in turgor pressure, generally <0.02 MPa, elicit increases in growth rate as predicted by the growth equations and the augmented growth equations. The irreversible wall extensibility and the yield threshold were determined from these results. The results also demonstrate that steps-up in turgor pressure larger than 0.02 MPa, produce a different response; a decrease in growth rate. The decreased growth rate behavior is related to the magnitude of the step-up, and in general, larger steps-up in turgor pressure produce larger decreases in growth rate and longer periods of decreased growth rate. Qualitatively, this growth behavior is very similar to the "stretch response" previously reported by Dennison and Roth (1967).