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Biophysical Journal 56: 683-691 (1989)
© 1989 the Biophysical Society

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Interaction of water with oriented DNA in the A- and B-form conformations.

R Brandes, A Rupprecht and D R Kearns

Department of Chemistry, University of California, San Diego, La Jolla 92093-0342.

ABSTRACT

High resolution 2H nuclear magnetic resonance (NMR) was used to investigate the interaction of D2O with solid samples of uniaxially oriented Li-DNA (B-form DNA) and Na-DNA (A- and B-form DNA). At low levels of hydration, 0 approximately 4 D2O/nucleotide, the 2H spectra shows a very weak (due to short T2) broad single resonance, suggestive of unrestricted rotational diffusion of the water. At approximately 5 or more D2O/nucleotide, the Li-DNA (B-form) spectra suddenly exhibit a large doublet splitting, characteristic of partially ordered water. With increasing hydration, the general trend is a decrease of this splitting. From our analysis we show that the DNA water structure reorganizes as the DNA is progressively hydrated. The D2O interaction with Na-DNA is rather different than with Li-DNA. Below 10 D2O/nucleotide Na-DNA is normally expected to be in the A-form, and a small, or negligible splitting is observed. In the range 9-19 D2O/nucleotide, the splitting increases with increasing hydration. Above approximately 20 D2O/nucleotide Na-DNA converts entirely to the B-form and the D2O splittings are then similar to those found in Li-DNA. We show that the complex Na-DNA results obtained in the range 0-20 D2O/nucleotide are caused by a mixture of A- and B-DNA in those samples.




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L. van Dam, N. Korolev, and L. Nordenskiold
Polyamine-nucleic acid interactions and the effects on structure in oriented DNA fibers
Nucleic Acids Res., January 15, 2002; 30(2): 419 - 428.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the Biophysical Society.