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Biophysical Journal 56: 851-859 (1989)
© 1989 the Biophysical Society

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Temperature jump study of charge translocation during the bacteriorhodopsin photocycle

Hans-Jürgen Butt, Klaus Fendler, András Dér * and Ernst Bamberg

Max-Planck-Institut für Biophysik, 6000 Frankfurt, Federal Republic of Germany
Institute of Biophysics, Biological Research Center, Hungarian Academy of Science, Szeged, Hungary

ABSTRACT

Temperature jump experiments were carried out on purple membranes oriented and fixed in polyacrylamide gel. With green background illumination a relaxation of the photocurrent after an infrared laser pulse could be observed. To simulate the temperature jump signals different models of the bacteriorhodopsin photocycle were tested. The parameters of these models were obtained by measuring absorbance changes and photocurrent after excitation with a 575-nm laser flash.

A model with a temperature-dependent branching before the M state turned out to be satisfying. Other models, especially those with a late branching or without branching, could not reproduce the temperature jump measurements.






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Copyright © 1989 by the Biophysical Society.