help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 57: 173-182 (1990)
© 1990 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaler, K V
Right arrow Articles by Jones, T B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaler, K V
Right arrow Articles by Jones, T B

Dielectrophoretic spectra of single cells determined by feedback-controlled levitation.

K V Kaler and T B Jones

Department of Electrical Engineering, University of Calgary, Alberta, Canada.

ABSTRACT

In this paper we have utilized the principle of dielectrophoresis (DEP) to develop an apparatus to stably levitate single biological cells using a digital feedback control scheme. Using this apparatus, the positive DEP spectra of both Canola plant protoplast and ligament fibroblast cells have been measured over a wide range of frequencies (1 kHz to 50 MHz) and suspending medium conductivities (11-800 muS/cm). The experimental data thus obtained have been interpreted in terms of a simple spherical cell model. Furthermore, utilizing such a model, we have shown that various cellular parameters of interest can be readily obtained from the measured DEP levitation spectrum. Specifically, the effective membrane capacitance of single cells has been determined. Values of 0.47 +/- 0.03 muF/cm2 for Canola protoplasts and 1.52 +/- 0.26 muF/cm2 for ligament fibroblasts thus obtained are consistent with those determined by other existing electrical methods.




This article has been cited by other articles:


Home page
 Biophys. JHome page
V. L. Sukhorukov, J. M. Endter, D. Zimmermann, R. Shirakashi, S. Fehrmann, M. Kiesel, R. Reuss, D. Becker, R. Hedrich, E. Bamberg, et al.
Mechanisms of Electrically Mediated Cytosolic Ca2+ Transients in Aequorin-Transformed Tobacco Cells
Biophys. J., November 1, 2007; 93(9): 3324 - 3337.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1990 by the Biophysical Society.