Biophysical Journal 58: 857-872 (1990)
© 1990 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cozens-Roberts, C Articles by Lauffenburger, D A Search for Related Content PubMed PubMed Citation Articles by Cozens-Roberts, C Articles by Lauffenburger, D A

Receptor-mediated cell attachment and detachment kinetics. II. Experimental model studies with the radial-flow detachment assay.

Department of Chemical Engineering, University of Pennsylvania, Philadelphia 19104.

ABSTRACT

Quantitative information regarding the kinetics of receptor-mediated cell adhesion to a ligand-coated surface are crucial for understanding the role of certain key parameters in many physiological and biotechnology-related processes. Here, we use the probabilistic attachment and detachment models developed in the preceding paper to interpret transient data from well-defined experiments. These data are obtained with a simple model cell system that consists of receptor-coated latex beads (prototype cells) and a Radial-Flow Detachment Assay (RFDA) using a ligand-coated glass disc. The receptors and ligands used in this work are complementary antibodies. The beads enable us to examine transient behavior with particles that possess fairly uniform properties that can be varied systematically, and the RFDA is designed for direct observation of adhesion to the ligand-coated glass surface over a range of shear stresses. Our experiments focus on the effects of surface shear stress, receptor density, and ligand density. These data provide a crucial test of the probabilistic framework. We show that these data can be explained with the probabilistic analyses, whereas they cannot be readily interpreted on the basis of a deterministic analysis. In addition, we examine transient data on cell adhesion reported from other assays, demonstrating the consistency of these data with the predictions of the probabilistic models.

This article has been cited by other articles:

				M. B. Dainiak, A. Kumar, I. Yu. Galaev, and B. Mattiasson Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel PNAS, January 24, 2006; 103(4): 849 - 854. [Abstract] [Full Text] [PDF]

				J. H Chon and E. L. Chaikof Soluble heparin-binding peptides regulate chemokinesis and cell adhesive forces Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1394 - C1402. [Abstract] [Full Text] [PDF]