Biophysical Journal 58: 1547-1555 (1990)
© 1990 the Biophysical Society

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Thermodynamical and structural information on photosynthetic systems obtained from electroluminescence kinetics

Department of Biophysics, Huygens Laboratory of the State University, 2300 RA Leiden, The Netherlands

ABSTRACT

The relationship between the thermodynamical and structural properties of photosynthetic reaction centers and kinetics and polarization of electric field-induced luminescence was studied. A general model is presented to describe the influence of an electric field on the individual electron transfer rate constants. Comparison of simulations with this model and experimental curves of Photosystem I electroluminescence showed that (a) at least three electrogenic electron transfer steps occur: P-700 to A₀(30%), A₀ to A₁ (50%), and A₁ to F_A(20%), (b) the midpoint potential of A₁/A-₁ is - 0.81 V, and (c) the emission moments of the pigments make on average an angle of 67° with the membrane normal. It is concluded that the analysis of electro-luminescence kinetics may be a powerful technique to obtain information on primary processes using relatively intact systems.

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