Biophysical Journal 59: 357-362 (1991)
© 1991 the Biophysical Society

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Vesicle sizing

Number distributions by dynamic light scattering

F. R. Hallett ^*, J. Watton ^* and P. Krygsman 

Guelph-Waterloo Program for Graduate Work in Physics, Department of Physics, University of Guelph, Guelph, Ontario N1G 2W1
Guelph-Waterloo Centre for Graduate Work in Chemistry, Department of Chemistry, University of Guelph, Guelph, Ontario N1G 2W1 Canada

ABSTRACT

A procedure is described which optimizes nonnegative least squares and exponential sampling fitting methods for analysis of dynamic light scattering (DLS) data from aqueous suspensions of vesicle/liposome systems. This approach utilizes a Rayleigh-Gans-Debye form factor for a coated sphere and yields number distributions which can be compared directly to distributions obtained by freeze-fracture electron microscopy (EM). Excellent agreement between the DLS and EM results are obtained for vesicle size distributions in the 100-200-nm range.

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