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Biophysical Journal 59: 889-893 (1991)
© 1991 the Biophysical Society

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Ionic channels in Langmuir-Blodgett films imaged by a scanning tunneling microscope.

O V Kolomytkin, A O Golubok, D N Davydov, V A Timofeev, S A Vinogradova and Tipisev SYa

Institute of Biophysics, Academy of Sciences of the USSR, Pushchino.

ABSTRACT

The molecular structure of channels formed by gramicidin A in a lipid membrane was imaged by a scanning tunneling microscope operating in air. The mono- and bimolecular films of lipid with gramicidin A were deposited onto a highly oriented pyrolitic graphite substrate by the Langmuir-Blodgett technique. It has been shown that under high concentration gramicidin A molecules can form in lipid films a quasi-regular, densely packed structure. Single gramicidin A molecules were imaged for the first time as well. The cavity of 0.4 +/- 0.05 nm in halfwidth was found on the scanning tunneling microscopy image of the gramicidin A molecule. The results of direct observation obtained by means of scanning tunneling microscope are in good agreement with the known molecular model of gramicidin A. It was shown that gramicidin A molecules can exist in a lipid monolayer as individual molecules or combined into clusters. The results demonstrate that scanning tunneling microscope can be used for high spatial resolution study of ionic channel structure.




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Copyright © 1991 by the Biophysical Society.