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Biophysical Journal 59: 939-944 (1991)
© 1991 the Biophysical Society

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Measurement of voltage dependence of capacitance of planar bilayer lipid membrane with a patch clamp amplifier.

S Toyama, A Nakamura and F Toda

Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Japan.

ABSTRACT

The voltage dependence of capacitance was measured by using the setup which was almost the same as that for the study of ion channels. The coefficient which represents the voltage dependence of capacitance itself also changes as a function of the duration of voltage application if hexadecane is contained in bilayer lipid membrane (BLM). The method of Alvarez, O., and R. Latorre (1978. Biophys. J. 21:1-17) was extended to treat BLM with hexadecane.







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Copyright © 1991 by the Biophysical Society.