help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 6: 153-162 (1966)
© 1966 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Painter, R. B.
Right arrow Articles by Ginoza, H. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Painter, R. B.
Right arrow Articles by Ginoza, H. S.

Some Characteristics of the Resistance Transfer Factor (RTF) Episome as Determined by Inactivation with Tritium, P32, and Gamma Radiation

Robert B. Painter and Herbert S. Ginoza

ABSTRACT

The resistance transfer factor (RTF) episome was studied by measuring its inactivation by Co60 gamma radiation, by incorporated P32, and by tritium incorporated as tritium-labeled thymine. The D37 for Co60 irradiation was 7 to 9 x 104 rad. Growth of the bacteria harboring the RTF in BUdR (bromouracil deoxyriboside) increased the sensitivity of the RTF to the gamma radiation. The RTF was markedly inactivated by tritium after growth of the host (thymine requiring) bacteria in tritium-labeled thymine, thus further establishing the presence of thymine in the genome of the RTF. Assuming the efficiency of inactivation by P32 to be 10%, the phosphorus content of the RTF was estimated to be about 2 x 105 P atoms/episome. The data suggest the RTF contains double stranded DNA with a molecular weight of the order of 3 to 8 x 107.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1966 by the Biophysical Society.