Biophysical Journal 6: 373-383 (1966)
© 1966 the Biophysical Society

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Studies of Chloroplast Development in Euglena

XV. Factors Influencing the Decay of Photoreactivability of Green Colony Formation

ABSTRACT

When UV-treated cells of Euglena gracilis var. bacillaris are incubated in the dark in a nutrient medium which permits cell division, they lose the ability to be photoreactivated. The rate of this loss increases with the UV dose. For any given UV dose, the rate of decay increases with increasing growth rate. The same phenomena are observed in light-grown and in dark-grown cells, although the sensitivity to UV of the light-grown cells is smaller by a factor of 1.7. The kinetics of photoreactivation (PR) change during the decay of photoreactivability only if the cells are incubated in growth medium. A UV-inactivation curve for cells photoreactivated only after appreciable PR shows the same slope as that for untreated cells (number of UV-sensitive targets). These results are discussed from the point of view of possible models.