Biophysical Journal 61: 956-962 (1992)
© 1992 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Linden, K G Articles by Jay, D G Search for Related Content PubMed PubMed Citation Articles by Linden, K G Articles by Jay, D G

Spatial specificity of chromophore assisted laser inactivation of protein function.

Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.

ABSTRACT

Chromophore assisted laser inactivation (CALI) is a new technique that selectively inactivates proteins of interest to elucidate their in vivo functions. This method has application to a wide array of biological questions. An understanding of aspects of the mechanism of CALI is required for its judicious application. A critical concern for CALI is its spatial specificity because nonspecific inactivation of neighboring unbound proteins by CALI is a possibility. We show here that CALI is very dependent on the distance between the chromophore and the protein such that there is no significant effect beyond 60 A. CALI using antibodies can inactivate other proteins through a complex but its efficacy decreases approximately fourfold for each intervening protein. These data imply that CALI is spatially specific and damage to neighboring proteins is unlikely.

This article has been cited by other articles:

				J. Guo, H. Chen, H. L. Puhl III, and S. R. Ikeda Fluorophore-assisted light inactivation produces both targeted and collateral effects on N-type calcium channel modulation in rat sympathetic neurons J. Physiol., October 15, 2006; 576(2): 477 - 492. [Abstract] [Full Text] [PDF]

				Y. Nakai and H. Kamiguchi Migration of nerve growth cones requires detergent-resistant membranes in a spatially defined and substrate-dependent manner J. Cell Biol., December 23, 2002; 159(6): 1097 - 1108. [Abstract] [Full Text] [PDF]

				T. Surrey, M. B. Elowitz, P.-E. Wolf, F. Yang, F. Nedelec, K. Shokat, and S. Leibler Chromophore-assisted light inactivation and self-organization of microtubules and motors PNAS, April 14, 1998; 95(8): 4293 - 4298. [Abstract] [Full Text] [PDF]