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Biophysical Journal 61: 1076-1086 (1992)
© 1992 the Biophysical Society

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Theoretical study of the multiline EPR signal from the S2 state of the oxygen evolving complex of photosystem II

Evidence for a magnetic tetramer

Jacques Bonvoisin *, Geneviève Blondin {ddagger}, Jean-Jacques Girerd {ddagger} and Jean-Luc Zimmermann §

Centre d'Elaboration de Matériaux et d'Etudes Structurales, Laboratoire d'Optique Electronique, CNRS UPR A 8011, B.P. 4347, 29 rue Jeanne Marvig, 31055 Toulouse
Laboratoire de Chimie Inorganique, Institut de Chimie Moléculaire d'Orsay, CNRS URA 420, Université de Paris Sud, 91405 Orsay
Section de Bioénergétique, Département de Biologie Cellulaire et Moléculaire, Centre d'Etudes de Saclay, 91191 Gif-sur-Yvette, France

ABSTRACT

The Oxygen evolving complex of plant photosystem II is made of a manganese cluster that gives rise to a low temperature EPR multiline signal in the S2 oxidation state. The origin of this EPR signal has been addressed with respect to the question of the magnetic couplings between the electron and nuclear spins of the four possible Mn ions that make up this complex. Considering Mn(III) and Mn(IV) as the only possible oxidation states present in the S2 state, and no large anisotropy of the magnetic tensors, the breadths of the EPR spectra calculated for dimers and trimers with S = 1/2 have been compared with that of the biological site. It is concluded that neither a dinuclear nor a trinuclear complex made of Mn(III) and Mn(IV) can be responsible for the multiline signal; but that, by contrast, a tetranuclear Mn complex can be the origin of this signal. The general shape of the experimental spectrum, its particular hyperfine pattern, the positions of most of the hyperfine lines and their relative intensities can be fit by a tetramer model described by the following six fitting parameters: g {approx} 1.987, A1 {approx} 122.4 10-4 cm-1, A2 {approx} 87.2 10-4 cm-1, A3 {approx} 81.6 10-4 cm-1, A4 {approx} 19.1 10-4 cm-1 and {delta}H = 24.5 G. A second model described by parameters very close to those given above except for A4 {approx} 77.5 10-4 cm-1 gives an equally good fit. However, no other set of parameters gives an EPR spectrum that reproduces the hyperfine pattern of the S2 multiline signal. This demonstrates that in the S2 state of the oxygen evolving complex, the four manganese ions are organized in a magnetic tetramer.






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Copyright © 1992 by the Biophysical Society.