| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 63: 35-57 (1992)
© 1992 the Biophysical Society
School of Chemical Engineering, Cornell University, Ithaca, New York 14853.
ABSTRACT
The receptor-mediated adhesion of cells to ligand-coated surfaces in viscous shear flow is an important step in many physiological processes, such as the neutrophil-mediated inflammatory response, lymphocyte homing, and tumor cell metastasis. This paper describes a calculational method which simulates the interaction of a single cell with a ligand-coated surface under flow. The cell is idealized as a microvilli-coated hard sphere covered with adhesive springs. The distribution of microvilli on the cell surface, the distribution of receptors on microvilli tips, and the forward and reverse reaction between receptor and ligand are all simulated using random number sampling of appropriate probability functions. The velocity of the cell at each time step in the simulation results from a balance of hydrodynamic, colloidal and bonding forces; the bonding force is derived by summing the individual contributions of each receptor-ligand tether. The model can simulate the effect of many parameters on adhesion, such as the number of receptors on microvilli tips, the density of ligand, the rates of reaction between receptor and ligand, the stiffness of the resulting receptor-ligand springs, the response of springs to strain, and the magnitude of the bulk hydrodynamic stresses. The model can successfully recreate the entire range of expected and observed adhesive phenomena, from completely unencumbered motion, to rolling, to transient attachment, to firm adhesion. Also, the method can generate meaningful statistical measures of adhesion, including the mean and variance in velocity, rate constants for cell attachment and detachment, and the frequency of adhesion. We find a critical modulating parameter of adhesion is the fractional spring slippage, which relates the strain of a bond to its rate of breakage; the higher the slippage, the faster the breakage for the same strain. Our analysis of neutrophil adhesive behavior on selectin-coated (CD62-coated) surfaces in viscous shear flow reported by Lawrence and Springer (Lawrence, M.B., and T.A. Springer 1991. Cell. 65:859-874) shows the fractional spring slippage of the CD62-LECAM-1 bond is likely below 0.01. We conclude the unique ability of this selectin bond to cause neutrophil rolling under flow is a result of its unique response to strain. Furthermore, our model can successfully recreate data on neutrophil rolling as function of CD62 surface density.
This article has been cited by other articles:
 |
|
 |
|
  A. D. Trister and D. A. Hammer Role of gp120 Trimerization on HIV Binding Elucidated with Brownian Adhesive Dynamics Biophys. J., July 1, 2008; 95(1): 40 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Caputo, D. Lee, M. R. King, and D. A. Hammer Adhesive Dynamics Simulations of the Shear Threshold Effect for Leukocytes Biophys. J., February 1, 2007; 92(3): 787 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. N. Everett, H.-J. Wu, S. G. Anekal, H.-J. Sue, and M. A. Bevan Diffusing Colloidal Probes of Protein and Synthetic Macromolecule Interactions Biophys. J., February 1, 2007; 92(3): 1005 - 1013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yu and J.-Y. Shao Simultaneous Tether Extraction Contributes to Neutrophil Rolling Stabilization: A Model Study Biophys. J., January 15, 2007; 92(2): 418 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. F. Krasik, K. L. Yee, and D. A. Hammer Adhesive Dynamics Simulation of Neutrophil Arrest with Deterministic Activation Biophys. J., August 15, 2006; 91(4): 1145 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Bayas, A. Leung, E. Evans, and D. Leckband Lifetime Measurements Reveal Kinetic Differences between Homophilic Cadherin Bonds Biophys. J., February 15, 2006; 90(4): 1385 - 1395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Sulchek, R. W. Friddle, K. Langry, E. Y. Lau, H. Albrecht, T. V. Ratto, S. J. DeNardo, M. E. Colvin, and A. Noy Dynamic force spectroscopy of parallel individual Mucin1-antibody bonds PNAS, November 15, 2005; 102(46): 16638 - 16643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. O. Eniola, E. F. Krasik, L. A. Smith, G. Song, and D. A. Hammer I-Domain of Lymphocyte Function-Associated Antigen-1 Mediates Rolling of Polystyrene Particles on ICAM-1 under Flow Biophys. J., November 1, 2005; 89(5): 3577 - 3588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Caputo and D. A. Hammer Effect of Microvillus Deformability on Leukocyte Adhesion Explored Using Adhesive Dynamics Simulations Biophys. J., July 1, 2005; 89(1): 187 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. English and D. A. Hammer The Effect of Cellular Receptor Diffusion on Receptor-Mediated Viral Binding Using Brownian Adhesive Dynamics (BRAD) Simulations Biophys. J., March 1, 2005; 88(3): 1666 - 1675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sun and L. L. Munn Particulate Nature of Blood Determines Macroscopic Rheology: A 2-D Lattice Boltzmann Analysis Biophys. J., March 1, 2005; 88(3): 1635 - 1645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jadhav, C. D. Eggleton, and K. Konstantopoulos A 3-D Computational Model Predicts that Cell Deformation Affects Selectin-Mediated Leukocyte Rolling Biophys. J., January 1, 2005; 88(1): 96 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. English and D. A. Hammer Brownian Adhesive Dynamics (BRAD) for Simulating the Receptor-Mediated Binding of Viruses Biophys. J., June 1, 2004; 86(6): 3359 - 3372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sun, C. Migliorini, and L. L. Munn Red Blood Cells Initiate Leukocyte Rolling in Postcapillary Expansions: A Lattice Boltzmann Analysis Biophys. J., July 1, 2003; 85(1): 208 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Doggett, G. Girdhar, A. Lawshe, J. L. Miller, I. J. Laurenzi, S. L. Diamond, and T. G. Diacovo Alterations in the intrinsic properties of the GPIb{alpha}-VWF tether bond define the kinetics of the platelet-type von Willebrand disease mutation, Gly233Val Blood, July 1, 2003; 102(1): 152 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Abbitt and G. B. Nash Rheological properties of the blood influencing selectin-mediated adhesion of flowing leukocytes Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H229 - H240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Migliorini, Y. Qian, H. Chen, E. B. Brown, R. K. Jain, and L. L. Munn Red Blood Cells Augment Leukocyte Rolling in a Virtual Blood Vessel Biophys. J., October 1, 2002; 83(4): 1834 - 1841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sheikh, R. Parhar, and F. Al-Mohanna Rapid static adhesion of human naive neutrophil to naive xenoendothelium under physiologic flow is independent of Gal{alpha}1,3-gal structures J. Leukoc. Biol., June 1, 2002; 71(6): 932 - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. King and D. A. Hammer Multiparticle adhesive dynamics: Hydrodynamic recruitment of rolling leukocytes PNAS, December 18, 2001; 98(26): 14919 - 14924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Topham, M. R. Castrucci, F. S. Wingo, G. T. Belz, and P. C. Doherty The Role of Antigen in the Localization of Naive, Acutely Activated, and Memory CD8+ T Cells to the Lung During Influenza Pneumonia J. Immunol., December 15, 2001; 167(12): 6983 - 6990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Fors, K. Goodarzi, and U. H. von Andrian L-Selectin Shedding Is Independent of Its Subsurface Structures and Topographic Distribution J. Immunol., October 1, 2001; 167(7): 3642 - 3651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-C. Chang, D. F. J. Tees, and D. A. Hammer The state diagram for cell adhesion under flow: Leukocyte rolling and firm adhesion PNAS, September 22, 2000; (2000) 200240897. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Weber, H. P. Wendel, and G. Ziemer Quality Assessment of Heparin Coatings by their Binding Capacities of Coagulation and Complement Enzymes J Biomater Appl, July 1, 2000; 15(1): 8 - 22. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Hafezi-Moghadam and K. Ley Relevance of L-selectin Shedding for Leukocyte Rolling In Vivo J. Exp. Med., March 15, 1999; 189(6): 939 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chen and T. A. Springer An Automatic Braking System That Stabilizes Leukocyte Rolling by an Increase in Selectin Bond Number with Shear J. Cell Biol., January 11, 1999; 144(1): 185 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Stein, G. Cheng, B. M. Stockton, B. P. Fors, E. C. Butcher, and U. H. von Andrian L-selectin-mediated Leukocyte Adhesion In Vivo: Microvillous Distribution Determines Tethering Efficiency, But Not Rolling Velocity J. Exp. Med., January 4, 1999; 189(1): 37 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Barber, A. Pinero, and G. A. Truskey Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells Am J Physiol Heart Circ Physiol, August 1, 1998; 275(2): H591 - H599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Shao, H. P. Ting-Beall, and R. M. Hochmuth Static and dynamic lengths of neutrophil microvilli PNAS, June 9, 1998; 95(12): 6797 - 6802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Alon, S. Chen, K. D. Puri, E. B. Finger, and T. A. Springer The Kinetics of L-selectin Tethers and the Mechanics of Selectin-mediated Rolling J. Cell Biol., September 8, 1997; 138(5): 1169 - 1180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen, R. Alon, R. C. Fuhlbrigge, and T. A. Springer Rolling and transient tethering of leukocytes on antibodies reveal specializations of selectins PNAS, April 1, 1997; 94(7): 3172 - 3177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Lawrence, G. S. Kansas, E. J. Kunkel, and K. Ley Threshold Levels of Fluid Shear Promote Leukocyte Adhesion through Selectins (CD62L,P,E) J. Cell Biol., February 10, 1997; 136(3): 717 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Smolen, T. K. Petersen, C. Koch, S. J. O'Keefe, W. A. Hanlon, S. Seo, D. Pearson, M. C. Fossett, and S. I. Simon L-Selectin Signaling of Neutrophil Adhesion and Degranulation Involves p38 Mitogen-activated Protein Kinase J. Biol. Chem., May 19, 2000; 275(21): 15876 - 15884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-C. Chang, D. F. J. Tees, and D. A. Hammer The state diagram for cell adhesion under flow: Leukocyte rolling and firm adhesion PNAS, October 10, 2000; 97(21): 11262 - 11267. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
