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Biophysical Journal 63: 323-326 (1992)
© 1992 the Biophysical Society

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Optical and physical properties of wet-spun films of Na-hyaluronate. Evidence of a phase transition.

M R Flowers, R L Marlow, S A Lee, N Lavalle and A Rupprecht

Department of Physics and Astronomy, University of Toledo, Ohio 43606.

ABSTRACT

The refractive indices, water content, and volume of wet-spun films of Na-hyaluronate have been measured as a function of relative humidity (rh). These data are used with the Lorentz-Lorenz formula to determine the optical polarizabilities of Na-hyaluronate parallel and perpendicular to the helical axis. The analysis reveals a drop in the optical polarizabilities of approximately 20% between 80 and 88% rh, indicating a phase transition.






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Copyright © 1992 by the Biophysical Society.