Biophysical Journal 63: 333-339 (1992)
© 1992 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thieffry, M Articles by Henry, J P Search for Related Content PubMed PubMed Citation Articles by Thieffry, M Articles by Henry, J P

Properties of the mitochondrial peptide-sensitive cationic channel studied in planar bilayers and patches of giant liposomes.

Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif sur Yvette, France.

ABSTRACT

A voltage-dependent cationic channel of large conductance is observed in phospholipid bilayers formed by the tip-dip method from proteoliposomes derived from mitochondrial membranes. It is blocked by peptide M, a 13 residue peptide having the properties of a mitochondrial signal sequence. To verify the reliability of the experimental approach, mitochondrial membranes from bovine adrenal cortex or porin-deficient mutant yeast were either fused to planar bilayers or incorporated in giant liposomes which were studied by patch clamp. Cationic channels were found with both techniques. They had the same conductance levels and voltage-dependence as those which have been described using the tip-dip method. Moreover, they were similarly blocked by peptide M. The voltage-dependence of block duration was analyzed in planar bilayer and tip-dip records. Results strengthen the idea that peptide M might cross the channel. Other mitochondrial channels were observed in planar bilayers and patch clamp of giant liposomes. Because they were never detected in tip-dip records, they are likely to be inactivated at the surface monolayer used to form the bilayer in this type of experiment.

This article has been cited by other articles:

				M. Poynor, R. Eckert, and S. Nussberger Dynamics of the Preprotein Translocation Channel of the Outer Membrane of Mitochondria Biophys. J., August 1, 2008; 95(3): 1511 - 1522. [Abstract] [Full Text] [PDF]

				S. Zhang, E. Udho, Z. Wu, R. J. Collier, and A. Finkelstein Protein Translocation through Anthrax Toxin Channels Formed in Planar Lipid Bilayers Biophys. J., December 1, 2004; 87(6): 3842 - 3849. [Abstract] [Full Text] [PDF]

				C. Muro, S. M. Grigoriev, D. Pietkiewicz, K. W. Kinnally, and M. L. Campo Comparison of the TIM and TOM Channel Activities of the Mitochondrial Protein Import Complexes Biophys. J., May 1, 2003; 84(5): 2981 - 2989. [Abstract] [Full Text] [PDF]

				U. Ahting, M. Thieffry, H. Engelhardt, R. Hegerl, W. Neupert, and S. Nussberger Tom40, the Pore-forming Component of the Protein-conducting TOM Channel in the Outer Membrane of Mitochondria J. Cell Biol., June 4, 2001; 153(6): 1151 - 1160. [Abstract] [Full Text] [PDF]

				K.-P. Kunkele, P. Juin, C. Pompa, F. E. Nargang, J.-P. Henry, W. Neupert, R. Lill, and M. Thieffry The Isolated Complex of the Translocase of the Outer Membrane of Mitochondria. CHARACTERIZATION OF THE CATION-SELECTIVE AND VOLTAGE-GATED PREPROTEIN-CONDUCTING PORE J. Biol. Chem., November 20, 1998; 273(47): 31032 - 31039. [Abstract] [Full Text] [PDF]

				I. Szabo, G. Bathori, F. Tombola, M. Brini, A. Coppola, and M. Zoratti DNA Translocation Across Planar Bilayers Containing Bacillus subtilis Ion Channels J. Biol. Chem., October 3, 1997; 272(40): 25275 - 25282. [Abstract] [Full Text] [PDF]

				T. A. Lohret, R. E. Jensen, and K. W. Kinnally Tim23, a Protein Import Component of the Mitochondrial Inner Membrane, Is Required for Normal Activity of the Multiple Conductance Channel, MCC J. Cell Biol., April 21, 1997; 137(2): 377 - 386. [Abstract] [Full Text] [PDF]

				P. Juin, M. Thieffry, J.-P. Henry, and F. M. Vallette Relationship between the Peptide-sensitive Channel and the Mitochondrial Outer Membrane Protein Translocation Machinery J. Biol. Chem., February 28, 1997; 272(9): 6044 - 6050. [Abstract] [Full Text] [PDF]

				T. A. Lohret and K. W. Kinnally Targeting Peptides Transiently Block a Mitochondrial Channel J. Biol. Chem., July 7, 1995; 270(27): 15950 - 15953. [Abstract] [Full Text] [PDF]

				E. V. Pavlov, M. Priault, D. Pietkiewicz, E. H.-Y. Cheng, B. Antonsson, S. Manon, S. J. Korsmeyer, C. A. Mannella, and K. W. Kinnally A novel, high conductance channel of mitochondria linked to apoptosis in mammalian cells and Bax expression in yeast J. Cell Biol., November 26, 2001; 155(5): 725 - 732. [Abstract] [Full Text] [PDF]