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Biophysical Journal 63: 1455-1461 (1992)
© 1992 the Biophysical Society

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Rod-like cholesterol micelles in aqueous solution studied using polarized and depolarized dynamic light scattering.

M A Castanho, W Brown and M J Prieto

Centro de Quimica Fisica Molecular, Lisboa, Portugal.

ABSTRACT

Micelles of cholesterol in aqueous solution have been investigated using polarized and depolarized dynamic light scattering. They are shown to be highly extended and characterized by a narrow size distribution. It is shown that a rod-like model is applicable with length, L = 580 nm. Determination of the rotational diffusion coefficient by analysis of the autocorrelation function gave a value of theta = 150 s-1, which is close to the calculated value for the rod with this dimension. Depolarized dynamic light scattering measurements as a function of angle gave a value of 110 s-1.




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Copyright © 1992 by the Biophysical Society.