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Biophysical Journal 64: 525-532 (1993)
© 1993 the Biophysical Society

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Elemental composition of secretory granules in pancreatic islets of Langerhans.

M C Foster, R D Leapman, M X Li and I Atwater

Department of Anesthesiology, School of Medicine, State University of New York, Stony Brook 11794-8480.

ABSTRACT

We have characterized, by electron probe microanalysis, rapidly frozen cultured rat islets at the level of individual secretory granules. Elemental analysis of thin, dried cryosections showed that beta granules could be distinguished by high Zn, Ca, and S, whereas non-beta (mainly alpha) granules contained elevated P and Mg. Although a single granule type predominated in a particular cell, some rebel granules were found in A cells that had the compositional fingerprint of B cell granules. Zn, which was found in millimolar concentrations in B cell granules, was considered a marker for the insulin storage complex. The data indicate that non-B islet cells in the adult pancreas may produce insulin-containing organelles and that, when glucagon and insulin are coexpressed, these hormones are packaged in separate granules.




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