| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 64: 716-723 (1993)
© 1993 the Biophysical Society
Department of Biochemistry and Biophysics, University of California, Davis 95616.
ABSTRACT
Fourier transform infrared spectroscopy is used to characterize specific interactions between negatively charged lipids, such as phosphatidic acid, and the purified nicotinic acetylcholine receptor from Torpedo californica. The specific interaction of phosphatidic acid with acetylcholine receptor is demonstrated by the receptor-induced perturbation of the lipid ionization state, which is monitored using Fourier transform infrared bands arising from the phosphate head group. The acetylcholine receptor shifts the pKa of phosphatidic acid molecules adjacent to the receptor to a lower value by almost 2 pH units from 8.5 to 6.6. Decreased pH also leads to changes in ion channel function and to changes in the secondary structure of the acetylcholine receptor in membranes containing ionizable phospholipids. Phospholipase D restores functional activity of acetylcholine receptor reconstituted in an unfavorable environment containing phosphatidylcholine by generating phosphatidic acid. Lipids such as phosphatidic acid may serve as allosteric effectors for membrane protein function and the lipid-protein interface could be a site for activity-dependent changes that lead to modulation of synaptic efficacy.
This article has been cited by other articles:
 |
|
 |
|
  C. J. B. daCosta, A. A. Ogrel, E. A. McCardy, M. P. Blanton, and J. E. Baenziger Lipid-Protein Interactions at the Nicotinic Acetylcholine Receptor. A FUNCTIONAL COUPLING BETWEEN NICOTINIC RECEPTORS AND PHOSPHATIDIC ACID-CONTAINING LIPID BILAYERS J. Biol. Chem., January 4, 2002; 277(1): 201 - 208. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. G. Eckenhoff PROMISCUOUS LIGANDS AND ATTRACTIVE CAVITIES: How do the inhaled anesthetics work? Mol. Interv., December 1, 2001; 1(5): 258 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Santiago, G. R. Guzman, L. V. Rojas, R. Marti, G. A. Asmar-Rovira, L. F. Santana, M. McNamee, and J. A. Lasalde-Dominicci Probing the Effects of Membrane Cholesterol in the Torpedo californica Acetylcholine Receptor and the Novel Lipid-exposed Mutation alpha C418W in Xenopus Oocytes J. Biol. Chem., November 30, 2001; 276(49): 46523 - 46532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Baenziger, M.-L. Morris, T. E. Darsaut, and S. E. Ryan Effect of Membrane Lipid Composition on the Conformational Equilibria of the Nicotinic Acetylcholine Receptor J. Biol. Chem., January 14, 2000; 275(2): 777 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Baro, R. M. Levini, M. T. Kim, A. R. Willms, C. C. Lanning, H. E. Rodriguez, and R. M. Harris-Warrick Quantitative Single-Cell-Reverse Transcription-PCR Demonstrates That A-Current Magnitude Varies as a Linear Function of shal Gene Expression in Identified Stomatogastric Neurons J. Neurosci., September 1, 1997; 17(17): 6597 - 6610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Ryan, C. N. Demers, J. P. Chew, and J. E. Baenziger Structural Effects of Neutral and Anionic Lipids on the Nicotinic Acetylcholine Receptor. AN INFRARED DIFFERENCE SPECTROSCOPY STUDY J. Biol. Chem., October 4, 1996; 271(40): 24590 - 24597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Antollini and F. J. Barrantes Unique Effects of Different Fatty Acid Species on the Physical Properties of the Torpedo Acetylcholine Receptor Membrane J. Biol. Chem., January 4, 2002; 277(2): 1249 - 1254. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
