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Biophysical Journal 64: 898-902 (1993)
© 1993 the Biophysical Society

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Modification of supported lipid membranes by atomic force microscopy

Susan L. Brandow, David C. Turner, Banahalli R. Ratna and Bruce P. Gaber

Center for Biomolecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5000 USA

ABSTRACT

The atomic force microscope (AFM) was used to structurally modify supported lipid bilayers in a controlled quantitative manner. By increasing the force applied by the AFM tip, lipid was removed from the scanned area, leaving a cut through the lipid bilayer. Cuts were repaired with the AFM by scanning the region with a controlled force and driving lipid back into the cut. A slow self-annealing of cuts was also observed.






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Copyright © 1993 by the Biophysical Society.