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Biophysical Journal 65: 1180-1187 (1993)
© 1993 the Biophysical Society

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Preparation of isolated biomolecules for SFM observations: T4 bacteriophage as a test sample.

E Droz, M Taborelli, T N Wells and P Descouts

Glaxo Institute for Molecular Biology, Geneva, Switzerland.

ABSTRACT

The T4 bacteriophage has been used to investigate protocols for the preparation of samples for scanning force microscopy in air, in order to obtaining reproducible images. The resolution of images and the distribution of bacteriophages on the substrate depends on the buffer type, its concentration, the surface treatment of substrate, and the method of deposition. The best imaging conditions for the phages require dilution in a volatile buffer at low ionic strength and adsorption onto hydrophilic surfaces. When imaging with the scanning force microscopy the quality of the images is influenced by the vertical and lateral forces applied on the sample and by the tip geometry.






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Copyright © 1993 by the Biophysical Society.