| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 65: 1590-1598 (1993)
© 1993 the Biophysical Society
Department of Physiology, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612.
ABSTRACT
Indirect evidence indicates that a proton-selective conductance is activated during the respiratory burst in neutrophils. A voltage- and time-dependent H(+)-selective conductance, gH, in human neutrophils is demonstrated here directly by the whole-cell patch-clamp technique. The gH is extremely low at large negative potentials, increases slowly upon membrane depolarization, and does not inactivate. It is enhanced at high external pH or low internal pH and is inhibited by Cd2+ and Zn2+. Arachidonic acid, which plays a pivotal role in inflammatory reactions, amplifies the gH. The properties of the gH described here are compatible with its activation during the respiratory burst in stimulated neutrophils, in which it may facilitate sustained superoxide anion release by dissipating metabolically generated acid.
This article has been cited by other articles:
 |
|
 |
|
  B. Musset, V. V. Cherny, D. Morgan, Y. Okamura, I. S. Ramsey, D. E. Clapham, and T. E. DeCoursey Detailed comparison of expressed and native voltage-gated proton channel currents J. Physiol., May 15, 2008; 586(10): 2477 - 2486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Morgan, V. V. Cherny, A. Finnegan, J. Bollinger, M. H. Gelb, and T. E. DeCoursey Sustained activation of proton channels and NADPH oxidase in human eosinophils and murine granulocytes requires PKC but not cPLA2{alpha} activity J. Physiol., March 1, 2007; 579(2): 327 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Femling, V. V. Cherny, D. Morgan, B. Rada, A. P. Davis, G. Czirjak, P. Enyedi, S. K. England, J. G. Moreland, E. Ligeti, et al. The Antibacterial Activity of Human Neutrophils and Eosinophils Requires Proton Channels but Not BK Channels J. Gen. Physiol., May 30, 2006; 127(6): 659 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Rada, M. Geiszt, K. Kaldi, C. Timar, and E. Ligeti Dual role of phagocytic NADPH oxidase in bacterial killing Blood, November 1, 2004; 104(9): 2947 - 2953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. DeCoursey During the Respiratory Burst, Do Phagocytes Need Proton Channels or Potassium Channels, or Both? Sci. Signal., May 18, 2004; 2004(233): pe21 - pe21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Petheo, A. Maturana, A. Spat, and N. Demaurex Interactions between Electron and Proton Currents in Excised Patches from Human Eosinophils J. Gen. Physiol., November 24, 2003; 122(6): 713 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Cherny, R. Murphy, V. Sokolov, R. A. Levis, and T. E. DeCoursey Properties of Single Voltage-gated Proton Channels in Human Eosinophils Estimated by Noise Analysis and by Direct Measurement J. Gen. Physiol., May 27, 2003; 121(6): 615 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Henderson and R. W. Meech Proton Conduction through gp91phox J. Gen. Physiol., November 25, 2002; 120(6): 759 - 765. [Full Text] [PDF]
|
|
|
|
|
|
N. Touret and S. Grinstein Voltage-gated Proton "Channels": a Spectator's Viewpoint J. Gen. Physiol., November 25, 2002; 120(6): 767 - 771. [Full Text] [PDF]
|
|
|
|
|
|
T. E. DeCoursey, D. Morgan, and V. V. Cherny The gp91phox Component of NADPH Oxidase Is Not a Voltage-gated Proton Channel J. Gen. Physiol., November 25, 2002; 120(6): 773 - 779. [Full Text] [PDF]
|
|
|
|
|
|
D. Morgan, V. V. Cherny, M. O. Price, M. C. Dinauer, and T. E. DeCoursey Absence of Proton Channels in COS-7 Cells Expressing Functional NADPH Oxidase Components J. Gen. Physiol., May 28, 2002; 119(6): 571 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Geiszt, A. Kapus, and E. Ligeti Chronic granulomatous disease: more than the lack of superoxide? J. Leukoc. Biol., February 1, 2001; 69(2): 191 - 196. [Abstract] [Full Text]
|
|
|
|
 |
|
 M.-L. Wu, C.-C. Chan, and M.-J. Su Possible Mechanism(s) of Arachidonic Acid-Induced Intracellular Acidosis in Rat Cardiac Myocytes Circ. Res., February 18, 2000; 86 (3): e55 - e62. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Bánfi, A. Maturana, S. Jaconi, S. Arnaudeau, T. Laforge, B. Sinha, E. Ligeti, N. Demaurex, and K. Krause A Mammalian H+ Channel Generated Through Alternative Splicing of the NADPH Oxidase Homolog NOH-1 Science, January 7, 2000; 287(5450): 138 - 142. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. E. DeCoursey Hypothesis: do voltage-gated H+ channels in alveolar epithelial cells contribute to CO2 elimination by the lung? Am J Physiol Cell Physiol, January 1, 2000; 278(1): C1 - C10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Banfi, J. Schrenzel, O. Nusse, D. P. Lew, E. Ligeti, K.-H. Krause, and N. Demaurex A Novel H+ Conductance in Eosinophils: Unique Characteristics and Absence in Chronic Granulomatous Disease J. Exp. Med., July 19, 1999; 190(2): 183 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Henderson Role of Histidines Identified by Mutagenesis in the NADPH Oxidase-associated H+ Channel J. Biol. Chem., December 11, 1998; 273(50): 33216 - 33223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Eder Ion channels in microglia (brain macrophages) Am J Physiol Cell Physiol, August 1, 1998; 275(2): C327 - C342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. T. Hii, Z. H. Huang, A. Bilney, M. Costabile, A. W. Murray, D. A. Rathjen, C. J Der, and A. Ferrante Stimulation of p38 Phosphorylation and Activity by Arachidonic Acid in HeLa Cells, HL60 Promyelocytic Leukemic Cells, and Human Neutrophils. EVIDENCE FOR CELL TYPE-SPECIFIC ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES J. Biol. Chem., July 24, 1998; 273(30): 19277 - 19282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Schumann, C. C. Leung, and T. A. Raffin Activation of NADPH-Oxidase and Its Associated Whole-cell H[IMAGE] Current in Human Neutrophils by Recombinant Human Tumor Necrosis Factor [IMAGE] and Formyl-Methionyl-Leucyl-Phenylalanine J. Biol. Chem., June 2, 1995; 270(22): 13124 - 13132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Henderson, G. Banting, and J. B. Chappell The Arachidonate-activable, NADPH Oxidase-associated H[IMAGE] Channel J. Biol. Chem., March 17, 1995; 270(11): 5909 - 5916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nordström, O. D. Rotstein, R. Romanek, S. Asotra, J. N. M. Heersche, M. F. Manolson, G. F. Brisseau, and S. Grinstein Regulation of Cytoplasmic pH in Osteoclasts J. Biol. Chem., February 3, 1995; 270(5): 2203 - 2212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. DeCoursey, V. V. Cherny, D. Morgan, B. Z. Katz, and M. C. Dinauer The gp91phox Component of NADPH Oxidase Is Not the Voltage-gated Proton Channel in Phagocytes, but It Helps J. Biol. Chem., September 21, 2001; 276(39): 36063 - 36066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Maturana, S. Arnaudeau, S. Ryser, B. Banfi, J. P. Hossle, W. Schlegel, K.-H. Krause, and N. Demaurex Heme Histidine Ligands within gp91phox Modulate Proton Conduction by the Phagocyte NADPH Oxidase J. Biol. Chem., August 3, 2001; 276(32): 30277 - 30284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. T. Hii, N. Moghadammi, A. Dunbar, and A. Ferrante Activation of the Phosphatidylinositol 3-Kinase-Akt/Protein Kinase B Signaling Pathway in Arachidonic Acid-stimulated Human Myeloid and Endothelial Cells. INVOLVEMENT OF THE ErbB RECEPTOR FAMILY J. Biol. Chem., July 13, 2001; 276(29): 27246 - 27255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. DeCoursey, V. V. Cherny, W. Zhou, and L. L. Thomas Simultaneous activation of NADPH oxidase-related proton and electron currents in human neutrophils PNAS, June 6, 2000; 97(12): 6885 - 6889. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
