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Biophysical Journal 66: 1180-1184 (1994)
© 1994 the Biophysical Society

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Measurement of the individual pKa values of acidic residues of hen and turkey lysozymes by two-dimensional 1H NMR.

K Bartik, C Redfield and C M Dobson

Chimie Organique E.P., Université Libre de Bruxelles, Belgium.

ABSTRACT

The pH dependence of the two-dimensional 1H nuclear magnetic resonance spectra of hen and turkey egg-white lysozymes has been recorded over the pH range 1-7. By monitoring the chemical shifts of the resonances of the various protons of ionizable residues, individual pKa values for the acidic residues have been determined for both proteins. The pKa values are displaced, with the exception of those of the residues in the active site cleft, by an average of 1 unit to low pH compared to model compounds.




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Copyright © 1994 by the Biophysical Society.