Biophysical Journal 66: 1328-1334 (1994)
© 1994 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fritz, M Articles by Gaub, H E Search for Related Content PubMed PubMed Citation Articles by Fritz, M Articles by Gaub, H E

Granula motion and membrane spreading during activation of human platelets imaged by atomic force microscopy.

Physik-Department, Technische Universität München, Garching, Germany.

ABSTRACT

The redistribution of platelet constituents during activation is essential for their physiological function of maintaining hemostasis. We report here about real time investigations of the activation of native human platelets under physiological conditions from the initial formation of filopodia to the fully spread form by atomic force microscopy. We followed the trafficking of granules and their interaction with the plasma membrane within single cells. Our results show movement of certain granula towards the lamellipodia. Analysis of this rearrangement and the subsequent enlargement of the platelet surface reveals details of the membrane spreading process. Images of living cells are presented that show the distribution of cytoskeletal components and membrane-bound filaments at a resolution of better than 50 nm. The local minimum forces between the tip and the platelets were estimated to be smaller than 60 pN. A model for the elastic contributions of the glycocalix to the tip/membrane interaction was developed using the theory of grafted polymers.

This article has been cited by other articles:

				K. Ghosh, S. Gangodkar, P. Jain, S. Shetty, S. Ramjee, P. Poddar, and A. Basu Imaging the interaction between dengue 2 virus and human blood platelets using atomic force and electron microscopy J. Electron Microsc. (Tokyo), June 1, 2008; 57(3): 113 - 118. [Abstract] [Full Text] [PDF]

				C. E. McNamee, N. Pyo, and K. Higashitani Atomic Force Microscopy Study of the Specific Adhesion between a Colloid Particle and a Living Melanoma Cell: Effect of the Charge and the Hydrophobicity of the Particle Surface Biophys. J., September 1, 2006; 91(5): 1960 - 1969. [Abstract] [Full Text] [PDF]

				I. D. Auerbach, C. Sorensen, H. G. Hansma, and P. A. Holden Physical Morphology and Surface Properties of Unsaturated Pseudomonas putida Biofilms J. Bacteriol., July 1, 2000; 182(13): 3809 - 3815. [Abstract] [Full Text]

				C. Rotsch, K. Jacobson, and M. Radmacher Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy PNAS, February 2, 1999; 96(3): 921 - 926. [Abstract] [Full Text] [PDF]

				E. Morgenstern; and S. W. Whiteheart The Pathway of Exocytosis in Human Platelets Blood, September 15, 1998; 92(6): 2191 - 2192. [Full Text] [PDF]

				S. W. Schneider, K. C. Sritharan, J. P. Geibel, H. Oberleithner, and B. P. Jena Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis PNAS, January 7, 1997; 94(1): 316 - 321. [Abstract] [Full Text] [PDF]

				M Radmacher, M Fritz, H. Hansma, and P. Hansma Direct observation of enzyme activity with the atomic force microscope Science, September 9, 1994; 265(5178): 1577 - 1579. [Abstract] [PDF]