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Biophysical Journal 67: 2394-2403 (1994)
© 1994 the Biophysical Society

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Reproducible acquisition of Escherichia coli porin surface topographs by atomic force microscopy.

F A Schabert and A Engel

Maurice E.-Müller Institute for Microscopic Structure Biology Biozentrum, University of Basel, Switzerland.

ABSTRACT

Crystalline membranes reconstituted from Escherichia coli OmpF porin and phospholipids were adsorbed to freshly cleaved mica and imaged in solution by the atomic force microscope. The extracellular as well as the periplasmic side of the porin trimers could be identified and the conditions to record topographs at 1-nm lateral and 0.1-nm vertical resolution were established.




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Copyright © 1994 by the Biophysical Society.