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Biophysical Journal 68: 1574-1582 (1995)
© 1995 the Biophysical Society

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Fluorescence lifetime-based sensing in tissues: a computational study.

C L Hutchinson, J R Lakowicz and E M Sevick-Muraca

Purdue University, School of Chemical Engineering, West Lafayette, Indiana 47907, USA.

ABSTRACT

We have numerically solved the photon diffusion equation to predict the distribution of light in a tissue model system with a uniform concentration of fluorophore. Our results show that time-dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a uniformly distributed fluorophore in tissues. With proper referencing, frequency-domain measurements of phase-shift, theta, may allow quantitation of fluorescent lifetimes, tau, independent of changes in the local absorption and scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetime, tau, when the lifetime of the fluorophore is comparable with photon migration times.




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A. Godavarty, M. J. Eppstein, C. Zhang, and E. M. Sevick-Muraca
Detection of Single and Multiple Targets in Tissue Phantoms with Fluorescence-enhanced Optical Imaging: Feasibility Study
Radiology, April 1, 2005; 235(1): 148 - 154.
[Abstract] [Full Text] [PDF]


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Copyright © 1995 by the Biophysical Society.