help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 7: 319-327 (1967)
© 1967 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rabinovitch, B.
Right arrow Articles by Weissbluth, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rabinovitch, B.
Right arrow Articles by Weissbluth, M.

Enzyme-Substrate Reactions in Very High Magnetic Fields. II

B. Rabinovitch, J. E. Maling and M. Weissbluth

ABSTRACT

We have examined the effect of conditioning the enzyme trypsin in solution at pH 8.2 in a large magnetic field before determining its reactivity towards a synthetic substrate N-benzoyl DL-arginine p-nitroanilide (BAPA). This "pretreatment" was allowed to proceed for as long as 32/3 hr in a magnetic field of 208 kgauss at temperatures 26 and 36°C. No effect on reactivity was observed when such pretreated enzyme solutions were compared with identical but untreated enzyme solutions. A single such reaction, allowed to proceed directly in a magnetic field of 220 kgauss for 9 min, similarly showed no difference in rate from its control.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1967 by the Biophysical Society.