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Biophysical Journal 7: 353-364 (1967)
© 1967 the Biophysical Society

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Cell Growth and Division

II. Experimental Studies of Cell Volume Distributions in Mammalian Suspension Cultures

Ernest C. Anderson and Donald F. Petersen

ABSTRACT

Experimental proof is given that the volume distribution spectrum of mammalian cells in suspension culture can be determined accurately with a Coulter spectrometer. Stable spectra corresponding to the predictions of a mathematical model are observed under favorable conditions of growth. Cell volume spectrometry appears to be a useful method for diagnosing the state of the culture with respect to past uniformity of growth rate and present population age distribution. In addition, it offers a method for quantitative study of the laws governing cell growth and division.






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Copyright © 1967 by the Biophysical Society.