Biophysical Journal 7: 375-389 (1967)
© 1967 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Papageorgiou, G. Articles by Govindjee, Search for Related Content PubMed PubMed Citation Articles by Papageorgiou, G. Articles by Govindjee,

Changes in Intensity and Spectral Distribution of Fluorescence

Effect of Light Treatment on Normal and DCMU^*-Poisoned Anacystis nidulans^,

ABSTRACT

The intensity of the "steady-state" fluorescence of "aerobic" Anacystis nidulans is variable under prolonged illumination with orange (590 mµ) or blue (440 mµ) light for both normally photosynthesizing and DCMU-poisoned cells. In general, orange light illumination causes an increase of the fluorescence intensity followed by a decrease, while blue light causes an increase until a steady level is reached. Poisoned Anacystis cells show four to eight times larger changes in fluorescence intensity than the normal cells; the detailed time course of fluorescence changes is also different in poisoned and normal cells. When algae are cooled to -196°C in light, the light-induced changes in the "steady-state" fluorescence disappear in both types of cells. Difference fluorescence spectra, constructed by subtracting the fluorescence spectra taken after 5-15 min of illumination from those after 60-90 min of illumination, show a doublet structure of the difference band with a major peak coinciding with the Anacystis emission maximum (685 mµ) and a minor peak located at about 693 mµ.

This article has been cited by other articles:

				T. Punnett Environmental Control of Photosynthetic Enhancement Science, January 22, 1971; 171(3968): 284 - 286. [Abstract] [PDF]