Biophysical Journal 70: 313-320 (1996)
© 1996 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Janzen, J Articles by Brooks, D E Search for Related Content PubMed PubMed Citation Articles by Janzen, J Articles by Brooks, D E

Interfacial thickness of liposomes containing poly(ethylene glycol)-cholesterol from electrophoresis.

Department of Pathology, University of British Columbia, Vancouver, Canada.

ABSTRACT

The electrophoretic mobilities of liposomes incorporating a polyethylene glycol (PEG) headgroup coupled to cholesterol for PEG of average chain index 3.0, 13.2, and 22.3 have been determined as a function of PEG-cholesterol mole fraction between 5% and 40% and ionic strength between 2 and 200 mM. The liposome compositions were 40 mole % cholesterol plus PEG-cholesterol, 10 mole % 1,2-dipalmitoyl-sn-glyerco-3-phosphoglycerol, and 50 mole % egg phosphatidylcholine. The mobilities were fit to a model in which the PEG forms a surface layer of polymer subject to viscous drag arising from electroosmotic flow within this layer. The model provides estimates of the average layer thickness that are comparable to those determined from contemporary models of surface-attached polymer.

This article has been cited by other articles:

				J. L. Swift and D. T. Cramb Nanoparticles as Fluorescence Labels: Is Size All that Matters? Biophys. J., July 15, 2008; 95(2): 865 - 876. [Abstract] [Full Text] [PDF]