Biophysical Journal 70: 1303-1315 (1996)
© 1996 the Biophysical Society

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by VanDongen, A M Search for Related Content PubMed PubMed Citation Articles by VanDongen, A M

A new algorithm for idealizing single ion channel data containing multiple unknown conductance levels.

Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA. vando005@mc.duke.edu

ABSTRACT

A new algorithm is presented for idealizing single channel data containing any number of conductance levels. The number of levels and their amplitudes do not have to be known a priori. No assumption has to be made about the behavior of the channel, other than that transitions between conductance levels are fast. The algorithm is relatively insensitive to the complexity of the underlying single channel behavior. Idealization may be reliable with signal-to-noise ratios as low as 3.5. The idealization algorithm uses a slope detector to localize transitions between levels and a relative amplitude criterion to remove spurious transitions. After estimating the number of conductances and their amplitudes, conductance states can be assigned to the idealized levels. In addition to improving the quality of the idealization, this "interpretation" allows a statistical analysis of individual (sub)conductance states.

This article has been cited by other articles:

				M. Poynor, R. Eckert, and S. Nussberger Dynamics of the Preprotein Translocation Channel of the Outer Membrane of Mitochondria Biophys. J., August 1, 2008; 95(3): 1511 - 1522. [Abstract] [Full Text] [PDF]

				M. L. Chapman and A. M.J. VanDongen K Channel Subconductance Levels Result from Heteromeric Pore Conformations J. Gen. Physiol., July 25, 2005; 126(2): 87 - 103. [Abstract] [Full Text] [PDF]

				T. Haug, R. Olcese, L. Toro, and E. Stefani Regulation of K+ Flow by a Ring of Negative Charges in the Outer Pore of BKCa Channels. Part II: Neutralization of Aspartate 292 Reduces Long Channel Openings and Gating Current Slow Component J. Gen. Physiol., July 26, 2004; 124(2): 185 - 197. [Abstract] [Full Text] [PDF]

				F. Qin Restoration of Single-Channel Currents Using the Segmental k-Means Method Based on Hidden Markov Modeling Biophys. J., March 1, 2004; 86(3): 1488 - 1501. [Abstract] [Full Text] [PDF]

				I. Dzhura and A. Neely Differential Modulation of Cardiac Ca2+ Channel Gating by {beta}-Subunits Biophys. J., July 1, 2003; 85(1): 274 - 289. [Abstract] [Full Text] [PDF]

				H. C. Lee, M. K. Patel, D. J. Mistry, Q. Wang, S. Reddy, J. R. Moorman, and J. P. Mounsey Abnormal Na channel gating in murine cardiac myocytes deficient in myotonic dystrophy protein kinase Physiol Genomics, January 15, 2003; 12(2): 147 - 157. [Abstract] [Full Text] [PDF]

				D. J. MISTRY, J. R. MOORMAN, S. REDDY, and J. P. MOUNSEY Skeletal muscle Na currents in mice heterozygous for Six5 deficiency Physiol Genomics, August 28, 2001; 6(3): 153 - 158. [Abstract] [Full Text] [PDF]

				P. Q. Barrett, H.-K. Lu, R. Colbran, A. Czernik, and J. J. Pancrazio Stimulation of unitary T-type Ca2+ channel currents by calmodulin-dependent protein kinase II Am J Physiol Cell Physiol, December 1, 2000; 279(6): C1694 - C1703. [Abstract] [Full Text] [PDF]

				J. P. Mounsey, D. J. Mistry, C. W. Ai, S. Reddy, and J. R. Moorman Skeletal muscle sodium channel gating in mice deficient in myotonic dystrophy protein kinase Hum. Mol. Genet., September 1, 2000; 9(15): 2313 - 2320. [Abstract] [Full Text] [PDF]

				Y. A. Kuryshev, G. M. Brittenham, H. Fujioka, P. Kannan, C.-C. Shieh, S. A. Cohen, and A. M. Brown Decreased Sodium and Increased Transient Outward Potassium Currents in Iron-Loaded Cardiac Myocytes : Implications for the Arrhythmogenesis of Human Siderotic Heart Disease Circulation, August 10, 1999; 100(6): 675 - 683. [Abstract] [Full Text] [PDF]

				J. Kiehn, A. E. Lacerda, and A. M. Brown Pathways of HERG inactivation Am J Physiol Heart Circ Physiol, July 1, 1999; 277(1): H199 - H210. [Abstract] [Full Text] [PDF]

				J.-H. Lee, A. N. Daud, L. L. Cribbs, A. E. Lacerda, A. Pereverzev, U. Klockner, T. Schneider, and E. Perez-Reyes Cloning and Expression of a Novel Member of the Low Voltage-Activated T-Type Calcium Channel Family J. Neurosci., March 15, 1999; 19(6): 1912 - 1921. [Abstract] [Full Text] [PDF]

				A. Alioua, Y. Tanaka, M. Wallner, F. Hofmann, P. Ruth, P. Meera, and L. Toro The Large Conductance, Voltage-dependent, and Calcium-sensitive K+ Channel, Hslo, Is a Target of cGMP-dependent Protein Kinase Phosphorylation in Vivo J. Biol. Chem., December 4, 1998; 273(49): 32950 - 32956. [Abstract] [Full Text] [PDF]

				J. W. Kramer, M. A. Post, A. M. Brown, and G. E. Kirsch Modulation of potassium channel gating by coexpression of Kv2.1 with regulatory Kv5.1 or Kv6.1 alpha -subunits Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1501 - C1510. [Abstract] [Full Text] [PDF]

				F. Romero, B. A. Silva, V. L. A. Nouailhetas, and J. Aboulafia Activation of Ca2+-activated K+ (maxi-K+) channel by angiotensin II in myocytes of the guinea pig ileum Am J Physiol Cell Physiol, April 1, 1998; 274(4): C983 - C991. [Abstract] [Full Text] [PDF]

				R. Dumaine and G. E. Kirsch Mechanism of lidocaine block of late current in long Q-T mutant Na+ channels Am J Physiol Heart Circ Physiol, February 1, 1998; 274(2): H477 - H487. [Abstract] [Full Text] [PDF]

				J. M. Pascual, C.-C. Shieh, G. E. Kirsch, and A. M. Brown Contribution of the NH2 terminus of Kv2.1 to channel activation Am J Physiol Cell Physiol, December 1, 1997; 273(6): C1849 - C1858. [Abstract] [Full Text] [PDF]

				E. A. Accili, J. Kiehn, B. A. Wible, and A. M. Brown Interactions among Inactivating and Noninactivating Kvbeta Subunits, and Kvalpha 1.2, Produce Potassium Currents with Intermediate Inactivation J. Biol. Chem., November 7, 1997; 272(45): 28232 - 28236. [Abstract] [Full Text] [PDF]

				E. A. Accili, J. Kiehn, Q. Yang, Z. Wang, A. M. Brown, and B. A. Wible Separable Kvbeta Subunit Domains Alter Expression and Gating of Potassium Channels J. Biol. Chem., October 10, 1997; 272(41): 25824 - 25831. [Abstract] [Full Text] [PDF]

				A. E. Lacerda, M.-L. Roy, E. W. Lewis, and D. Rampe Interactions of the Nonsedating Antihistamine Loratadine with a Kv1.5-Type Potassium Channel Cloned from Human Heart Mol. Pharmacol., August 1, 1997; 52(2): 314 - 322. [Abstract] [Full Text]

				T. J. DiChiara and P. H. Reinhart Redox Modulation of hslo Ca2+-Activated K+ Channels J. Neurosci., July 1, 1997; 17(13): 4942 - 4955. [Abstract] [Full Text] [PDF]

				J. Kiehn, A. E. Lacerda, B. Wible, and A. M. Brown Molecular Physiology and Pharmacology of HERG: Single-Channel Currents and Block by Dofetilide Circulation, November 15, 1996; 94(10): 2572 - 2579. [Abstract] [Full Text]