| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 71: 695-706 (1996)
© 1996 the Biophysical Society
Department of General Physiology, University of Ulm, Germany.
ABSTRACT
Voltage-dependent gating was investigated in a recombinant human skeletal muscle Cl- channel, hCIC-1, heterologously expressed in human embryonic kidney (HEK-293) cells. Gating was found to be mediated by two qualitatively distinct processes. One gating step operates on a microsecond time scale and involves the rapid rearrangement of two identical intramembranous voltage sensors, each consisting of a single titratable residue. The second process occurs on a millisecond time scale and is due to a blocking-unblocking reaction mediated by a cytoplasmic gate that interacts with the ion pore of the channel. These results illustrate a rather simple structural basis for voltage sensing that has evolved in skeletal muscle Cl- channels and provides evidence for the existence of a cytoplasmic gating mechanism in an anion channel analogous to the "ball and chain" mechanism of voltage-gated cation channels.
This article has been cited by other articles:
 |
|
 |
|
  J. A. de Santiago, K. Nehrke, and J. Arreola Quantitative Analysis of the Voltage-dependent Gating of Mouse Parotid ClC-2 Chloride Channel J. Gen. Physiol., November 28, 2005; 126(6): 591 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hebeisen and C. Fahlke Carboxy-Terminal Truncations Modify the Outer Pore Vestibule of Muscle Chloride Channels Biophys. J., September 1, 2005; 89(3): 1710 - 1720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-F. Wu, A. Ryan, J. Devaney, M. Warnstedt, Z. Korade-Mirnics, B. Poser, M. J. Escriva, E. Pegoraro, A. S. Yee, K. J. Felice, et al. Novel CLCN1 mutations with unique clinical and electrophysiological consequences Brain, November 1, 2002; 125(11): 2392 - 2407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Warnstedt, C. Sun, B. Poser, M. J. Escriva, L. Tranebjarg, T. Torbergsen, M. van Ghelue, and C. Fahlke The Myotonia Congenita Mutation A331T Confers a Novel Hyperpolarization-Activated Gate to the Muscle Chloride Channel ClC-1 J. Neurosci., September 1, 2002; 22(17): 7462 - 7470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cuppoletti, K. P. Tewari, A. M. Sherry, E. Y. Kupert, and D. H. Malinowska ClC-2 Cl{-} channels in human lung epithelia: activation by arachidonic acid, amidation, and acid-activated omeprazole Am J Physiol Cell Physiol, July 1, 2001; 281(1): C46 - C54. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Aromataris, G. Y. Rychkov, B. Bennetts, B. P. Hughes, A. H. Bretag, and M. L. Roberts Fast and Slow Gating of CLC-1: Differential Effects of 2-(4-Chlorophenoxy) Propionic Acid and Dominant Negative Mutations Mol. Pharmacol., July 1, 2001; 60(1): 200 - 208. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Fahlke Ion permeation and selectivity in ClC-type chloride channels Am J Physiol Renal Physiol, May 1, 2001; 280(5): F748 - F757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, S. Bendahhou, M. C. Sanguinetti, and L. J. Ptacek Functional consequences of chloride channel gene (CLCN1) mutations causing myotonia congenita Neurology, February 22, 2000; 54(4): 937 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, M. C. Sanguinetti, H. Kwiecinski, and L. J. Ptacek Mechanism of Inverted Activation of ClC-1 Channels Caused by a Novel Myotonia Congenita Mutation J. Biol. Chem., January 28, 2000; 275(4): 2999 - 3005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lehmann-Horn and K. Jurkat-Rott Voltage-Gated Ion Channels and Hereditary Disease Physiol Rev, October 1, 1999; 79(4): 1317 - 1372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Kurz, H. Klink, I. Jakob, M. Kuchenbecker, S. Benz, F. Lehmann-Horn, and R. Rudel Identification of Three Cysteines as Targets for the Zn2+ Blockade of the Human Skeletal Muscle Chloride Channel J. Biol. Chem., April 23, 1999; 274(17): 11687 - 11692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Stroffekova, E. Y. Kupert, D. H. Malinowska, and J. Cuppoletti Identification of the pH sensor and activation by chemical modification of the ClC-2G Cl- channel Am J Physiol Cell Physiol, October 1, 1998; 275(4): C1113 - C1123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Fahlke, C. L. Beck, and A. L. George Jr. A mutation in autosomal dominant myotonia congenita affects pore properties of the muscle chloride channel PNAS, March 18, 1997; 94(6): 2729 - 2734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Fahlke, R. R. Desai, N. Gillani, and A. L. George Jr. Residues Lining the Inner Pore Vestibule of Human Muscle Chloride Channels J. Biol. Chem., January 12, 2001; 276(3): 1759 - 1765. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
