| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 71: 2033-2039 (1996)
© 1996 the Biophysical Society
Department of Medical Biochemistry, Texas A & M University, College Station 77843-1114, USA.
ABSTRACT
It is clear that intramolecular hydrogen bonds are essential to the structure and stability of globular proteins. It is not clear, however, whether they make a net favorable contribution to this stability. Experimental and theoretical studies are at odds over this important question. Measurements of the change in conformational stability, delta (delta G), for the mutation of a hydrogen bonded residue to one incapable of hydrogen bonding suggest a stabilization of 1.0 kcal/mol per hydrogen bond. If the delta (delta G) values are corrected for differences in side-chain hydrophobicity and conformational entropy, then the estimated stabilization becomes 2.2 kcal/mol per hydrogen bond. These and other experimental studies discussed here are consistent and compelling: hydrogen bonding stabilizes globular proteins.
This article has been cited by other articles:
 |
|
 |
|
  J. Gao and J. W. Kelly Toward quantification of protein backbone-backbone hydrogen bonding energies: An energetic analysis of an amide-to-ester mutation in an {alpha}-helix within a protein Protein Sci., June 1, 2008; 17(6): 1096 - 1101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Wolf and S. W. de Leeuw Fast In Silico Protein Folding by Introduction of Alternating Hydrogen Bond Potentials Biophys. J., May 15, 2008; 94(10): 3742 - 3747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bernado, M. Blackledge, and J. Sancho Sequence-Specific Solvent Accessibilities of Protein Residues in Unfolded Protein Ensembles Biophys. J., December 15, 2006; 91(12): 4536 - 4543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Rose, P. J. Fleming, J. R. Banavar, and A. Maritan A backbone-based theory of protein folding PNAS, November 7, 2006; 103(45): 16623 - 16633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kongsaeree, P. Khongsuk, U. Leartsakulpanich, P. Chitnumsub, B. Tarnchompoo, M. D. Walkinshaw, and Y. Yuthavong Crystal structure of dihydrofolate reductase from Plasmodium vivax: Pyrimethamine displacement linked with mutation-induced resistance PNAS, September 13, 2005; 102(37): 13046 - 13051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Fleming and G. D. Rose Do all backbone polar groups in proteins form hydrogen bonds? Protein Sci., July 1, 2005; 14(7): 1911 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sinha, N. Mitra, G. Kumar, K. Bajaj, and A. Surolia Unfolding Studies on Soybean Agglutinin and Concanavalin A Tetramers: A Comparative Account Biophys. J., February 1, 2005; 88(2): 1300 - 1310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Campos, S. Cuesta-Lopez, J. Lopez-Llano, F. Falo, and J. Sancho A Double-Deletion Method to Quantifying Incremental Binding Energies in Proteins from Experiment: Example of a Destabilizing Hydrogen Bonding Pair Biophys. J., February 1, 2005; 88(2): 1311 - 1321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Lomize, I.D. Pogozheva, and H.I. Mosberg Quantification of helix-helix binding affinities in micelles and lipid bilayers Protein Sci., October 22, 2004; 13(10): 2600 - 2612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Peterson, P. L. Dutton, and A. J. Wand Improved side-chain prediction accuracy using an ab initio potential energy function and a very large rotamer library Protein Sci., March 1, 2004; 13(3): 735 - 751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fernandez and H. A. Scheraga Insufficiently dehydrated hydrogen bonds as determinants of protein interactions PNAS, January 7, 2003; 100(1): 113 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tareste, F. Pincet, E. Perez, S. Rickling, C. Mioskowski, and L. Lebeau Energy of Hydrogen Bonds Probed by the Adhesion of Functionalized Lipid Layers Biophys. J., December 1, 2002; 83(6): 3675 - 3681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Lomize, M. Y. Reibarkh, and I. D. Pogozheva Interatomic potentials and solvation parameters from protein engineering data for buried residues Protein Sci., August 1, 2002; 11(8): 1984 - 2000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Funahashi, K. Takano, Y. Yamagata, and K. Yutani Positive Contribution of Hydration Structure on the Surface of Human Lysozyme to the Conformational Stability J. Biol. Chem., June 7, 2002; 277(24): 21792 - 21800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ragone Hydrogen-bonding classes in proteins and their contribution to the unfolding reaction Protein Sci., October 19, 2001; 10(10): 2075 - 2082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Takano and K. Yutani A new scale for side-chain contribution to protein stability based on the empirical stability analysis of mutant proteins Protein Eng. Des. Sel., August 1, 2001; 14(8): 525 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Irun, S. Maldonado, and J. Sancho Stabilization of apoflavodoxin by replacing hydrogen-bonded charged Asp or Glu residues by the neutral isosteric Asn or Gln Protein Eng. Des. Sel., March 1, 2001; 14(3): 173 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Goldenberg, R. E. Koehn, D. E. Gilbert, and G. Wagner Solution structure and backbone dynamics of an {{omega}}-conotoxin precursor Protein Sci., March 1, 2001; 10(3): 538 - 550. [Abstract] [Full Text]
|
|
|
|
|
|
J. Funahashi, K. Takano, and K. Yutani Are the parameters of various stabilization factors estimated from mutant human lysozymes compatible with other proteins? Protein Eng. Des. Sel., February 1, 2001; 14(2): 127 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takano, M. Ota, K. Ogasahara, Y. Yamagata, K. Nishikawa, and K. Yutani Experimental verification of the `stability profile of mutant protein' (SPMP) data using mutant human lysozymes Protein Eng. Des. Sel., August 1, 1999; 12(8): 663 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Myers, C. N. Pace, and J. M. Scholtz A direct comparison of helix propensity in proteins and peptides PNAS, April 1, 1997; 94(7): 2833 - 2837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Aharoni and A. Horovitz Detection of changes in pairwise interactions during allosteric transitions: Coupling between local and global conformational changes in GroEL PNAS, March 4, 1997; 94(5): 1698 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Aurora, T. P. Creamer, R. Srinivasan, and G. D. Rose Local Interactions in Protein Folding: Lessons from the alpha -Helix J. Biol. Chem., January 17, 1997; 272(3): 1413 - 1416. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
