| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biophysical Journal 71: 2669-2679 (1996)
© 1996 the Biophysical Society
Physics Department, Rice University, Houston, Texas 77005-1892, USA.
ABSTRACT
Alamethicin adsorbs on the membrane surface at low peptide concentrations. However, above a critical peptide-to-lipid ratio (P/L), a fraction of the peptide molecules insert in the membrane. This critical ratio is lipid dependent. For diphytanoyl phosphatidylcholine it is about 1/40. At even higher concentrations P/L > or = 1/15, all of the alamethicin inserts into the membrane and forms well-defined pores as detected by neutron in-plane scattering. A previous x-ray diffraction measurement showed that alamethicin adsorbed on the surface has the effect of thinning the bilayer in proportion to the peptide concentration. A theoretical study showed that the energy cost of membrane thinning can indeed lead to peptide insertion. This paper extends the previous studies to the high-concentration region P/L > 1/40. X-ray diffraction shows that the bilayer thickness increases with the peptide concentration for P/L > 1/23 as the insertion approaches 100%. The thickness change with the percentage of insertion is consistent with the assumption that the hydrocarbon region of the bilayer matches the hydrophobic region of the inserted peptide. The elastic energy of a lipid bilayer including both adsorption and insertion of peptide is discussed. The Gibbs free energy is calculated as a function of P/L and the percentage of insertion phi in a simplified one-dimensional model. The model exhibits an insertion phase transition in qualitative agreement with the data. We conclude that the membrane deformation energy is the major driving force for the alamethicin insertion transition.
This article has been cited by other articles:
 |
|
 |
|
  M. Zoonens, Y. K. Reshetnyak, and D. M. Engelman Bilayer Interactions of pHLIP, a Peptide that Can Deliver Drugs and Target Tumors Biophys. J., July 1, 2008; 95(1): 225 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ringstad, E. Andersson Nordahl, A. Schmidtchen, and M. Malmsten Composition Effect on Peptide Interaction with Lipids and Bacteria: Variants of C3a Peptide CNY21 Biophys. J., January 1, 2007; 92(1): 87 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. A. Domanov and P. K. J. Kinnunen Antimicrobial Peptides Temporins B and L Induce Formation of Tubular Lipid Protrusions from Supported Phospholipid Bilayers Biophys. J., December 15, 2006; 91(12): 4427 - 4439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bakas, A. Chanturiya, V. Herlax, and J. Zimmerberg Paradoxical Lipid Dependence of Pores Formed by the Escherichia coli {alpha}-Hemolysin in Planar Phospholipid Bilayer Membranes Biophys. J., November 15, 2006; 91(10): 3748 - 3755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li and T. Salditt Structure of Magainin and Alamethicin in Model Membranes Studied by X-Ray Reflectivity Biophys. J., November 1, 2006; 91(9): 3285 - 3300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-T. Lee, W.-C. Hung, F.-Y. Chen, and H. W. Huang Many-Body Effect of Antimicrobial Peptides: On the Correlation Between Lipid's Spontaneous Curvature and Pore Formation Biophys. J., December 1, 2005; 89(6): 4006 - 4016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mecke, D.-K. Lee, A. Ramamoorthy, B. G. Orr, and M. M. Banaszak Holl Membrane Thinning Due to Antimicrobial Peptide Binding: An Atomic Force Microscopy Study of MSI-78 in Lipid Bilayers Biophys. J., December 1, 2005; 89(6): 4043 - 4050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Suarez, M. Haenni, S. Canarelli, F. Fisch, P. Chodanowski, C. Servis, O. Michielin, R. Freitag, P. Moreillon, and N. Mermod Structure-Function Characterization and Optimization of a Plant-Derived Antibacterial Peptide Antimicrob. Agents Chemother., September 1, 2005; 49(9): 3847 - 3857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Lensink, B. Christiaens, J. Vandekerckhove, A. Prochiantz, and M. Rosseneu Penetratin-Membrane Association: W48/R52/W56 Shield the Peptide from the Aqueous Phase Biophys. J., February 1, 2005; 88(2): 939 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Spaar, C. Munster, and T. Salditt Conformation of Peptides in Lipid Membranes Studied by X-Ray Grazing Incidence Scattering Biophys. J., July 1, 2004; 87(1): 396 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zemel, A. Ben-Shaul, and S. May Membrane Perturbation Induced by Interfacially Adsorbed Peptides Biophys. J., June 1, 2004; 86(6): 3607 - 3619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Buffy, T. Hong, S. Yamaguchi, A. J. Waring, R. I. Lehrer, and M. Hong Solid-State NMR Investigation of the Depth of Insertion of Protegrin-1 in Lipid Bilayers Using Paramagnetic Mn2+ Biophys. J., October 1, 2003; 85(4): 2363 - 2373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zemel, D. R. Fattal, and A. Ben-Shaul Energetics and Self-Assembly of Amphipathic Peptide Pores in Lipid Membranes Biophys. J., April 1, 2003; 84(4): 2242 - 2255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. P. Galbraith, R. Harris, P. C. Driscoll, and B. A. Wallace Solution NMR Studies of Antiamoebin, a Membrane Channel-Forming Polypeptide Biophys. J., January 1, 2003; 84(1): 185 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. P. Hamill and B. Martinac Molecular Basis of Mechanotransduction in Living Cells Physiol Rev, April 1, 2001; 81(2): 685 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Fisher, K. Campanale, B. L. Ackermann, M. VandenBranden, and S. A. Wrighton In Vitro Glucuronidation Using Human Liver Microsomes and The Pore-Forming Peptide Alamethicin Drug Metab. Dispos., May 1, 2000; 28(5): 560 - 566. [Abstract] [Full Text]
|
|
|
|
 |
|
 I. Peri, H. Mamrud-Brains, S. Rodin, V. Krizhanovsky, Y. Shai, S. Nir, and M. Naim Rapid entry of bitter and sweet tastants into liposomes and taste cells: implications for signal transduction Am J Physiol Cell Physiol, January 1, 2000; 278(1): C17 - C25. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
