help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Biophysical Journal 72: 117-126 (1997)
© 1997 the Biophysical Society

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krovetz, H S
Right arrow Articles by VanDongen, A M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krovetz, H S
Right arrow Articles by VanDongen, A M

Atomic distance estimates from disulfides and high-affinity metal-binding sites in a K+ channel pore.

H S Krovetz, H M VanDongen and A M VanDongen

Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA.

ABSTRACT

The pore of potassium channels is lined by four identical, highly conserved hairpin loops, symmetrically arranged around a central permeation pathway. Introduction of cysteines into the external mouth of the drk1 K channel pore resulted in the formation of disulfide bonds that were incompatible with channel function. Breaking these bonds restored function and resulted in a high-affinity Cd(2+)-binding site, indicating coordinated ligation by multiple sulfhydryls. Dimeric constructs showed that these disulfide bonds formed between subunits. These results impose narrow constraints on intersubunit atomic distances in the pore that strongly support a radial pore model. The data also suggest an important functional role for the outer mouth of the pore in gating or permeation.




This article has been cited by other articles:


Home page
 Biophys. JHome page
H.-S. Sun, K. Hui, D. W. K. Lee, and Z.-P. Feng
Zn2+ Sensitivity of High- and Low-Voltage Activated Calcium Channels
Biophys. J., August 15, 2007; 93(4): 1175 - 1183.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Liu, C. Alexander, J. Serrano, E. Borg, and D. C. Dawson
Variable Reactivity of an Engineered Cysteine at Position 338 in Cystic Fibrosis Transmembrane Conductance Regulator Reflects Different Chemical States of the Thiol
J. Biol. Chem., March 24, 2006; 281(12): 8275 - 8285.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Giorgetti, P. Carloni, P. Mistrik, and V. Torre
A Homology Model of the Pore Region of HCN Channels
Biophys. J., August 1, 2005; 89(2): 932 - 944.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
M. L. Chapman and A. M.J. VanDongen
K Channel Subconductance Levels Result from Heteromeric Pore Conformations
J. Gen. Physiol., July 25, 2005; 126(2): 87 - 103.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Horenstein, P. Riegelhaupt, and M. H. Akabas
Differential Protein Mobility of the {gamma}-Aminobutyric Acid, Type A, Receptor {alpha} and {beta} Subunit Channel-lining Segments
J. Biol. Chem., January 14, 2005; 280(2): 1573 - 1581.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Panicker, H. Cruz, C. Arrabit, K. F. Suen, and P. A. Slesinger
Minimal Structural Rearrangement of the Cytoplasmic Pore during Activation of the 5-HT3A Receptor
J. Biol. Chem., July 2, 2004; 279(27): 28149 - 28158.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
S. Kellenberger, I. Gautschi, and L. Schild
Mutations in the Epithelial Na+ Channel ENaC Outer Pore Disrupt Amiloride Block by Increasing Its Dissociation Rate
Mol. Pharmacol., October 1, 2003; 64(4): 848 - 856.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. J. Neale, D. J. S. Elliott, M. Hunter, and A. Sivaprasadarao
Evidence for Intersubunit Interactions between S4 and S5 Transmembrane Segments of the Shaker Potassium Channel
J. Biol. Chem., August 1, 2003; 278(31): 29079 - 29085.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
Y.-W. Lin, T. Jia, A. M. Weinsoft, and S.-L. Shyng
Stabilization of the Activity of ATP-sensitive Potassium Channels by Ion Pairs Formed between Adjacent Kir6.2 Subunits
J. Gen. Physiol., July 28, 2003; 122(2): 225 - 237.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. Mazzolini, M. Punta, and V. Torre
Movement of the C-Helix during the Gating of Cyclic Nucleotide-Gated Channels
Biophys. J., December 1, 2002; 83(6): 3283 - 3295.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. H. Aziz, C. J. Partridge, T. S. Munsey, and A. Sivaprasadarao
Depolarization Induces Intersubunit Cross-linking in a S4 Cysteine Mutant of the Shaker Potassium Channel
J. Biol. Chem., November 1, 2002; 277(45): 42719 - 42725.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P. Roncaglia, P. Mistrik, and V. Torre
Pore Topology of the Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel from Sea Urchin Sperm
Biophys. J., October 1, 2002; 83(4): 1953 - 1964.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Loussouarn, E. N. Makhina, T. Rose, and C. G. Nichols
Structure and Dynamics of the Pore of Inwardly Rectifying KATP Channels
J. Biol. Chem., January 14, 2000; 275(2): 1137 - 1144.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. C. DAWSON, S. S. SMITH, and M. K. MANSOURA
CFTR: Mechanism of Anion Conduction
Physiol Rev, January 1, 1999; 79(1): 47 - 75.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-S. Wu, H. D. Edwards, and W. A. Sather
Side Chain Orientation in the Selectivity Filter of a Voltage-gated Ca2+ Channel
J. Biol. Chem., October 6, 2000; 275(41): 31778 - 31785.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the Biophysical Society.