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Biophysical Journal 72: 545-553 (1997)
© 1997 the Biophysical Society

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In Vivo Photocycle of the Euglena gracilis Photoreceptor

Laura Barsanti *, Vincenzo Passarelli *, Patricia L. Walne # and Paolo Gualtieri *

Istituto di Biofisica, CNR, I-56127 Pisa, Italy
Department of Botany, University of Tennessee, Knoxville, Tennessee 37996 USA

ABSTRACT

We present the light-induced photocycle of the paraflagellar swelling of Euglena gracilis. The kinetics of this process was reconstructed by sampling its fluorescence emission and switching the excitation light from 365 nm to 436 nm. Stable intermediates in the photocycle were manifested. The measured millisecond resolution kinetics best fits a Michaelis-Menten equation. The data provide strong evidence that the paraflagellar swelling, a three-dimensional natural crystal of a light-detecting protein, is the true Euglena photoreceptor.






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Copyright © 1997 by the Biophysical Society.